Title |
Soluble RANKL expression in Lactococcus lactis and investigation of its potential as an oral vaccine adjuvant
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Published in |
BMC Immunology, November 2015
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DOI | 10.1186/s12865-015-0132-x |
Pubmed ID | |
Authors |
Jeong-In Kim, Tae-Eun Park, Sushila Maharjan, Hui-Shan Li, Ho-Bin Lee, In-Seon Kim, Dachuan Piao, Jun-Yeong Lee, Chong-Su Cho, Jin-Duck Bok, Zhong-Shan Hong, Sang-Kee Kang, Yun-Jaie Choi |
Abstract |
To initiate mucosal immune responses, antigens in the intestinal lumen must be transported into gut-associated lymphoid tissue through M cells. Recently, it has been increasingly recognized that receptor activator of NF-kB ligand (RANKL) controls M cell differentiation by interacting with RANK expressed on the sub-epithelium of Peyer's patches. In this study, we increased the number of M cells using soluble RANKL (sRANKL) as a potent mucosal adjuvant. For efficient oral delivery of sRANKL, we constructed recombinant Lactococcus lactis (L. lactis) IL1403 secreting sRANKL (sRANKL-LAB). The biological activity of recombinant sRANKL was confirmed by observing RANK-RANKL signaling in vitro. M cell development in response to oral administration of recombinant L. lactis was determined by 1.51-fold higher immunohistochemical expression of M cell marker GP-2, compared to that of non-treatment group. In addition, an adjuvant effect of sRANKL was examined by immunization of mice with M-BmpB as a model antigen after treatment with sRANKL-LAB. Compared with the wild-type L. lactis group, the sRANKL-LAB group showed significantly increased systemic and mucosal immune responses specific to M-BmpB. Our results show that the M cell development by sRANKL-LAB can increase the antigen transcytotic capability of follicle-associated epithelium, and thereby enhance the mucosal immune response, which implies that oral administration of sRANKL is a promising adjuvant strategy for efficient oral vaccination. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 19% |
Student > Ph. D. Student | 3 | 14% |
Student > Doctoral Student | 2 | 10% |
Student > Bachelor | 2 | 10% |
Student > Postgraduate | 2 | 10% |
Other | 6 | 29% |
Unknown | 2 | 10% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 29% |
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Medicine and Dentistry | 2 | 10% |
Engineering | 2 | 10% |
Social Sciences | 1 | 5% |
Other | 3 | 14% |
Unknown | 3 | 14% |