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Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays

Overview of attention for article published in BMC Veterinary Research, December 2017
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2 tweeters

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Title
Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays
Published in
BMC Veterinary Research, December 2017
DOI 10.1186/s12917-017-1284-0
Pubmed ID
Authors

Peili Hou, Hongmei Wang, Guimin Zhao, Chengqiang He, Hongbin He

Abstract

Infectious bovine rhinotracheitis virus (IBRV) is a major pathogen in cattle and has led to significant economic losses to the dairy industry worldwide, and therefore a more optimal method for the rapid diagnosis of IBRV infection is highly needed. In this study, we described the development of a lateral flow dipstrip (LFD) of isothermal recombinase polymerase amplification (RPA) method for rapid detection of IBRV. Distinct regions were selected as a candidate target for designing the LFD-RPA primers and probes. The analytical sensitivity of the RPA assay was determined using ten-fold serially diluted IBRV DNA. The specificity of the assay was assessed with other viral pathogens of cattle with similar clinic and other herpesviruses. The clinical performance was evaluated by testing 106 acute-phase high fever clinical specimens. RPA primers and probe were designed to target the specific conserved UL52 region fragment of IBRV. The detection could be completed at a constant temperature of 38 °C for 25 min, and the amplification products were easily visualized on a simple LFD. The detection limit of this assay was 5 copies per reaction of IBRV DNA and there was no cross-reactivity with other viruses causing bovine gastrointestinal and respiratory infections or other herpesviruses. The assay performance on acute-phase high fever clinical samples collected from cattle with no vaccine against IBRV, which were suspected to be infected with IBRV, was validated by detecting 24 fecal, 36 blood, 38 nasal swab and 8 tissue specimens, and compared with SYBR Green I based real-time PCR. The coincidence between IBRV LFD-RPA and real-time PCR was 100%. IBRV LFD-RPA was fast and much easier to serve as an alternative to the common measures used for IBRV diagnosis, as there is reduction in the use of instruments for identification of the infected animals. In addition, this assay may be the potential candidate to be used as point-of-care diagnostics in the field.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 23%
Student > Master 5 13%
Researcher 4 10%
Student > Doctoral Student 3 8%
Other 3 8%
Other 6 15%
Unknown 10 25%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 11 28%
Biochemistry, Genetics and Molecular Biology 8 20%
Agricultural and Biological Sciences 7 18%
Chemical Engineering 1 3%
Computer Science 1 3%
Other 1 3%
Unknown 11 28%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2017.
All research outputs
#9,823,551
of 12,299,992 outputs
Outputs from BMC Veterinary Research
#1,109
of 1,714 outputs
Outputs of similar age
#246,261
of 344,940 outputs
Outputs of similar age from BMC Veterinary Research
#105
of 146 outputs
Altmetric has tracked 12,299,992 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,714 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,940 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 146 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.