Title |
Collagen-derived dipeptide prolyl-hydroxyproline promotes osteogenic differentiation through Foxg1
|
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Published in |
Cellular & Molecular Biology Letters, December 2017
|
DOI | 10.1186/s11658-017-0060-2 |
Pubmed ID | |
Authors |
Yoshifumi Kimira, Haruka Odaira, Kaho Nomura, Yuri Taniuchi, Naoki Inoue, Sachie Nakatani, Jun Shimizu, Masahiro Wada, Hiroshi Mano |
Abstract |
Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. We previously reported that Pro-Hyp promotes the differentiation of osteoblasts by increasing Runx2, osterix and Col1α1 mRNA expression levels. Here, to elucidate the mechanism of Pro-Hyp promotion of osteoblast differentiation, we focus on the involvement of Foxo1 in osteoblast differentiation via Runx2 regulation and the role of Foxg1 in Foxo1 regulation. The addition of Pro-Hyp had no effect on MC3T3-E1 cell proliferation in Foxo1- or Foxg1-knockdown cells. In Foxo1-knockdown cells, the addition of Pro-Hyp increased ALP activity, but in Foxg1-knockdown cells, it had no effect on ALP activity. An enhancing effect of Pro-Hyp on the Runx2 and osterix expression levels was observed in Foxo1-knockdown cells. However, no enhancing effect of Pro-Hyp on osteoblastic gene expression was observed when Foxg1 was knocked down. These results demonstrate that Pro-Hyp promotes osteoblastic MC3T3-E1 cell differentiation and upregulation of osteogenic genes via Foxg1 expression. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 18% |
Professor | 2 | 12% |
Student > Ph. D. Student | 2 | 12% |
Student > Bachelor | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 7 | 41% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 12% |
Engineering | 2 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Computer Science | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Other | 2 | 12% |
Unknown | 8 | 47% |