Title |
Complete genome sequence of lytic bacteriophage RG-2014 that infects the multidrug resistant bacterium Delftia tsuruhatensis ARB-1
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Published in |
Environmental Microbiome, December 2017
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DOI | 10.1186/s40793-017-0290-y |
Pubmed ID | |
Authors |
Ananda Shankar Bhattacharjee, Amir Mohaghegh Motlagh, Eddie B. Gilcrease, Md Imdadul Islam, Sherwood R. Casjens, Ramesh Goel |
Abstract |
A lytic bacteriophage RG-2014 infecting a biofilm forming multidrug resistant bacterium Delftia tsuruhatensis strain ARB-1 as its host was isolated from a full-scale municipal wastewater treatment plant. Lytic phage RG-2014 was isolated for developing phage based therapeutic approaches against Delftia tsuruhatensis strain ARB-1. The strain ARB-1 belongs to the Comamonadaceae family of the Betaproteobacteria class. RG-2014 was characterized for its type, burst size, latent and eclipse time periods of 150 ± 9 PFU/cell, 10-min, <5-min, respectively. The phage was found to be a dsDNA virus belonging to the Podoviridae family. It has an isometric icosahedrally shaped capsid with a diameter of 85 nm. The complete genome of the isolated phage was sequenced and determined to be 73.8 kbp in length with a G + C content of 59.9%. Significant similarities in gene homology and order were observed between Delftia phage RG-2014 and the E. coli phage N4 indicating that it is a member of the N4-like phage group. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 33% |
United States | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 4 | 19% |
Student > Bachelor | 3 | 14% |
Researcher | 3 | 14% |
Student > Doctoral Student | 2 | 10% |
Lecturer | 1 | 5% |
Other | 4 | 19% |
Unknown | 4 | 19% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 4 | 19% |
Agricultural and Biological Sciences | 3 | 14% |
Biochemistry, Genetics and Molecular Biology | 2 | 10% |
Engineering | 2 | 10% |
Immunology and Microbiology | 1 | 5% |
Other | 3 | 14% |
Unknown | 6 | 29% |