Myra O. Villareal, Ayumi Ikeya, Kazunori Sasaki, Abdelkarim Ben Arfa, Mohamed Neffati, Hiroko Isoda
Abstract
Mood disorder accounts for 13 % of global disease burden. And while therapeutic agents are available, usually orally administered, most have unwanted side effects, and thus making the inhalation of essential oils (EOs) an attractive alternative therapy. Rosmarinus officinalis EO (ROEO), Mediterranean ROEO reported to improve cognition, mood, and memory, the effect on stress of which has not yet been determined. Here, the anti-stress effect of ROEO on stress was evaluated in vivo and in vitro.
Six-week-old male ICR mice were made to inhale ROEO and subjected to tail suspension test (TST). To determine the neuronal differentiation effect of ROEO in vitro, induction of ROEO-treated PC12 cells differentiation was observed. Intracellular acetylcholine and choline, as well as the Gap43 gene expression levels were also determined.
Inhalation of ROEO significantly decreased the immobility time of ICR mice and serum corticosterone level, accompanied by increased brain dopamine level. Determination of the underlying mechanism in vitro revealed a PC12 differentiation-induction effect through the modulation of intracellular acetylcholine, choline, and Gap43 gene expression levels. ROEO activates the stress response system through the NGF pathway and the hypothalamus-pituitary-adrenal axis, promoting dopamine production and secretion. The effect of ROEO may be attributed to its bioactive components, specifically to α-pinene, one of its major compounds that has anxiolytic property.
The results of this study suggest that ROEO inhalation has therapeutic potential against stress-related psychiatric disorders.
Pharmacology, Toxicology and Pharmaceutical Science
12
12%
Agricultural and Biological Sciences
8
8%
Biochemistry, Genetics and Molecular Biology
4
4%
Neuroscience
4
4%
Other
12
12%
Unknown
50
49%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 209. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2023.
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#185,538
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Outputs from BMC Complementary Medicine and Therapies
#34
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Outputs of similar age
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Outputs of similar age from BMC Complementary Medicine and Therapies
#2
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Altmetric has tracked 25,359,594 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
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