Title |
Quantification of cell identity from single-cell gene expression profiles
|
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Published in |
Genome Biology, January 2015
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DOI | 10.1186/s13059-015-0580-x |
Pubmed ID | |
Authors |
Idan Efroni, Pui-Leng Ip, Tal Nawy, Alison Mello, Kenneth D Birnbaum |
Abstract |
The definition of cell identity is a central problem in biology. While single-cell RNA-seq provides a wealth of information regarding cell states, better methods are needed to map their identity, especially during developmental transitions. Here, we use repositories of cell type-specific transcriptomes to quantify identities from single-cell RNA-seq profiles, accurately classifying cells from Arabidopsis root tips and human glioblastoma tumors. We apply our approach to single cells captured from regenerating roots following tip excision. Our technique exposes a previously uncharacterized transient collapse of identity distant from the injury site, demonstrating the biological relevance of a quantitative cell identity index. |
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United States | 6 | 24% |
India | 3 | 12% |
France | 2 | 8% |
Australia | 1 | 4% |
China | 1 | 4% |
Peru | 1 | 4% |
United Kingdom | 1 | 4% |
Switzerland | 1 | 4% |
Germany | 1 | 4% |
Other | 1 | 4% |
Unknown | 7 | 28% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 12 | 48% |
Scientists | 11 | 44% |
Science communicators (journalists, bloggers, editors) | 2 | 8% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 2 | <1% |
United States | 2 | <1% |
Chile | 1 | <1% |
Switzerland | 1 | <1% |
United Kingdom | 1 | <1% |
Australia | 1 | <1% |
Unknown | 272 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 69 | 25% |
Researcher | 54 | 19% |
Student > Master | 26 | 9% |
Student > Postgraduate | 20 | 7% |
Student > Bachelor | 15 | 5% |
Other | 51 | 18% |
Unknown | 45 | 16% |
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Agricultural and Biological Sciences | 128 | 46% |
Biochemistry, Genetics and Molecular Biology | 62 | 22% |
Computer Science | 13 | 5% |
Neuroscience | 6 | 2% |
Medicine and Dentistry | 5 | 2% |
Other | 17 | 6% |
Unknown | 49 | 18% |