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Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP

Overview of attention for article published in Parasites & Vectors, January 2015
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21 Mendeley
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Title
Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP
Published in
Parasites & Vectors, January 2015
DOI 10.1186/s13071-015-0673-0
Pubmed ID
Authors

Qiang Miao, Si-Yang Huang, Si-Yuan Qin, Xin Yu, Yan Yang, Jian-Fa Yang, Xing-Quan Zhu, Feng-Cai Zou

Abstract

Background Toxoplasma gondii is a protozoan parasite that infects almost all warm-blooded animals and human beings. Goats are one of the susceptible animals to T. gondii. However, little is known of genetic diversity of T. gondii in Yunnan black goats in China. The objective of this present study was to determine the genotypes of T. gondii isolates from black goats in Yunnan province, southwest China.MethodsGenomic DNA was extracted from liver (n¿=¿403), lung (n¿=¿403) and lymph nodes (n¿=¿250) of Yunnan black goats and assayed for T. gondii infection by semi-nested PCR of B1 gene. Then, the positive DNA samples were typed at 10 genetic markers using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. These markers include 9 nuclear loci, namely, SAG1, SAG2 (5¿-SAG2 and 3¿-SAG2, alternative SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast locus Apico.ResultsOut of 403 tested samples, 20 (4.96%) DNA samples were T. gondii positive by amplification of B1 gene. Among them, 2 isolates were genotyped at all loci, and 6 isolates were genotyped for 8 or more loci. In total, seven samples belong to ToxoDB PCR-RFLP genotype#10 (Type I), and one belongs to genotype ToxoDB #9.ConclusionsTo our knowledge, this is the first report of ToxoDB#9 and ToxoDB#10¿T. gondii in Yunnan black goats in China. These results revealed a wide distribution of these T. gondii in Yunnan black goats in China, which has important implications for public health.

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The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 1 5%
Unknown 20 95%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 29%
Student > Doctoral Student 4 19%
Student > Ph. D. Student 4 19%
Student > Bachelor 2 10%
Professor 1 5%
Other 3 14%
Unknown 1 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 38%
Medicine and Dentistry 4 19%
Veterinary Science and Veterinary Medicine 3 14%
Immunology and Microbiology 3 14%
Social Sciences 1 5%
Other 1 5%
Unknown 1 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 January 2015.
All research outputs
#3,142,062
of 4,689,255 outputs
Outputs from Parasites & Vectors
#802
of 1,327 outputs
Outputs of similar age
#105,415
of 162,213 outputs
Outputs of similar age from Parasites & Vectors
#63
of 106 outputs
Altmetric has tracked 4,689,255 research outputs across all sources so far. This one is in the 29th percentile – i.e., 29% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,327 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
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We're also able to compare this research output to 106 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.