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Mendeley readers
Attention Score in Context
| Title |
Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002
|
|---|---|
| Published in |
Journal of Biological Engineering, June 2017
|
| DOI | 10.1186/s13036-017-0061-8 |
| Pubmed ID | |
| Authors |
Anne Ilse Maria Vogel, Rahmi Lale, Martin Frank Hohmann-Marriott |
| Abstract |
Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus chromosome, validation of neutral integration sites with optimization of the DNA transformation protocol parameters is necessary. Availability of BioBrick-compatible integration modules is desirable to further simplifying chromosomal integrations. We designed three BioBrick-compatible genetic modules, each targeting a separate neutral integration site, A2842, A0935, and A0159, with varying length of homologous region, spanning from 100 to 800 nt. The performance of the different modules for achieving DNA integration were tested. Our results demonstrate that 100 nt homologous regions are sufficient for inserting a 1 kb DNA fragment into the Synechococcus chromosome. By adapting a transformation protocol from a related cyanobacterium, we shortened the transformation procedure for Synechococcus significantly. The optimized transformation protocol reported in this study provides an efficient way to perform genetic engineering in Synechococcus. We demonstrated that homologous regions of 100 nt are sufficient for inserting a 1 kb DNA fragment into the three tested neutral integration sites. Integration at A2842, A0935 and A0159 results in only a minimal fitness cost for the chassis. This study contributes to developing Synechococcus as the prominent chassis for future synthetic biology applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 69 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 22% |
| Student > Bachelor | 10 | 14% |
| Student > Master | 9 | 13% |
| Researcher | 8 | 12% |
| Student > Doctoral Student | 6 | 9% |
| Other | 6 | 9% |
| Unknown | 15 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 29 | 42% |
| Agricultural and Biological Sciences | 10 | 14% |
| Chemical Engineering | 5 | 7% |
| Unspecified | 2 | 3% |
| Engineering | 2 | 3% |
| Other | 3 | 4% |
| Unknown | 18 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2022.
All research outputs
#7,711,992
of 23,452,723 outputs
Outputs from Journal of Biological Engineering
#122
of 273 outputs
Outputs of similar age
#121,749
of 318,255 outputs
Outputs of similar age from Journal of Biological Engineering
#3
of 5 outputs
Altmetric has tracked 23,452,723 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 273 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 318,255 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.