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A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus

Overview of attention for article published in Virology Journal, June 2010
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (64th percentile)
  • Good Attention Score compared to outputs of the same age and source (68th percentile)

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1 X user
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7 Wikipedia pages

Citations

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15 Dimensions

Readers on

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43 Mendeley
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Title
A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus
Published in
Virology Journal, June 2010
DOI 10.1186/1743-422x-7-113
Pubmed ID
Authors

Xiao-ping Kang, Tao Jiang, Yong-qiang Li, Fang Lin, Hong Liu, Guo-hui Chang, Qing-yu Zhu, E-de Qin, Cheng-feng Qin, Yin-hui Yang

Abstract

A duplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay was improved for simultaneous detection of highly pathogenic H5N1 avian influenza virus and pandemic H1N1 (2009) influenza virus, which is suitable for early diagnosis of influenza-like patients and for epidemiological surveillance. The sensitivity of this duplex real-time RT-PCR assay was 0.02 TCID50 (50% tissue culture infective dose) for H5N1 and 0.2 TCID50 for the pandemic H1N1, which was the same as that of each single-target RT-PCR for pandemic H1N1 and even more sensitive for H5N1 with the same primers and probes. No cross reactivity of detecting other subtype influenza viruses or respiratory tract viruses was observed. Two hundred and thirty-six clinical specimens were tested by comparing with single real-time RT-PCR and result from the duplex assay was 100% consistent with the results of single real-time RT-PCR and sequence analysis.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Indonesia 1 2%
United States 1 2%
Denmark 1 2%
Unknown 40 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 28%
Student > Master 8 19%
Student > Bachelor 6 14%
Professor > Associate Professor 3 7%
Student > Doctoral Student 2 5%
Other 7 16%
Unknown 5 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 42%
Medicine and Dentistry 6 14%
Immunology and Microbiology 5 12%
Biochemistry, Genetics and Molecular Biology 3 7%
Veterinary Science and Veterinary Medicine 2 5%
Other 4 9%
Unknown 5 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 February 2022.
All research outputs
#7,404,945
of 23,305,591 outputs
Outputs from Virology Journal
#877
of 3,094 outputs
Outputs of similar age
#33,783
of 97,297 outputs
Outputs of similar age from Virology Journal
#8
of 22 outputs
Altmetric has tracked 23,305,591 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,094 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.8. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 97,297 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.