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Expression of hypoxia inducible factor 1α and 2α and its association with vitamin C level in thyroid lesions

Overview of attention for article published in Journal of Biomedical Science, October 2017
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Title
Expression of hypoxia inducible factor 1α and 2α and its association with vitamin C level in thyroid lesions
Published in
Journal of Biomedical Science, October 2017
DOI 10.1186/s12929-017-0388-y
Pubmed ID
Authors

Paweł Jóźwiak, Piotr Ciesielski, Agnieszka Zaczek, Anna Lipińska, Lech Pomorski, Marek Wieczorek, Magdalena Bryś, Ewa Forma, Anna Krześlak

Abstract

Cells adapt to hypoxia by transcriptional induction of genes that participate in regulation of angiogenesis, glucose metabolism and cell proliferation. The primary factors mediating cell response to low oxygen tension are hypoxia inducible factors (HIFs), oxygen-dependent transcription activators. The stability and activity of the α subunits of HIFs are controlled by hydroxylation reactions that require ascorbate as a cofactor. Therefore, deficiency of intracellular vitamin C could contribute to HIFs overactivation. In this study, we investigated whether vitamin C content of human thyroid lesions is associated with HIF-1α and HIF-2α protein levels. Expression of HIF-1α and HIF-2α as well as vitamin C content was analyzed in thyroid lesions and cultured thyroid carcinoma cell lines (FTC-133 and 8305c) treated with hypoxia-mimetic agent (cobalt chloride) and ascorbic acid. The expression of HIFs and hypoxia-induced glucose transporters were determined by Western blots while quantitative real-time PCR (qRT-PCR) was performed to detect HIFs mRNA levels. Ascorbate and dehydroascorbate levels were measured by HPLC method. We found an inverse correlation between vitamin C level and HIF-1α but not HIF-2α expression in thyroid lesions. These results agree with our in vitro study showing that vitamin C induced a dose - dependent decrease of HIF-1α but not HIF-2α protein level in thyroid cancer cells FTC-133 and 8305C. The decreased HIF-1α expression was correlated with reduced expression of hypoxia-related glucose transporter 1 (GLUT1) in thyroid cancer cells. The results demonstrate that HIF-1α activation is associated with vitamin C content in thyroid lesions. Our study suggests that high tumor tissue ascorbate level could limit the expression of HIF-1α and its targets in thyroid lesions.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 17%
Student > Master 4 13%
Student > Bachelor 3 10%
Researcher 3 10%
Professor 2 7%
Other 3 10%
Unknown 10 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 13%
Nursing and Health Professions 3 10%
Immunology and Microbiology 2 7%
Neuroscience 2 7%
Agricultural and Biological Sciences 2 7%
Other 4 13%
Unknown 13 43%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 July 2021.
All research outputs
#17,525,298
of 21,689,971 outputs
Outputs from Journal of Biomedical Science
#733
of 938 outputs
Outputs of similar age
#300,918
of 401,382 outputs
Outputs of similar age from Journal of Biomedical Science
#1
of 1 outputs
Altmetric has tracked 21,689,971 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 938 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.9. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
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