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Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs

Overview of attention for article published in Parasites & Vectors, January 2018
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  • Good Attention Score compared to outputs of the same age (70th percentile)

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1 policy source
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2 tweeters

Citations

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13 Dimensions

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50 Mendeley
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Title
Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs
Published in
Parasites & Vectors, January 2018
DOI 10.1186/s13071-017-2553-2
Pubmed ID
Authors

Santiago Radio, Santiago Fontenla, Victoria Solana, Anna C. Matos Salim, Flávio Marcos Gomes Araújo, Pedro Ortiz, Cristian Hoban, Estefan Miranda, Valeria Gayo, Fabiano Sviatopolk-Mirsky Pais, Hugo Solana, Guilherme Oliveira, Pablo Smircich, José F. Tort

Abstract

Fasciola hepatica is the main agent of fasciolosis, a zoonotic disease affecting livestock worldwide, and an emerging food-borne disease in humans. Even when effective treatments are available, drugs are costly and can result in tolerance, liver damage and normally they do not prevent reinfection. Drug-resistant strains in livestock have been reported in various countries and, more worryingly, drug resistance in human cases has emerged in South America. The present study aims to characterize the transcriptome of two South American resistant isolates, the Cajamarca isolate from Peru, resistant to both triclabendazole and albendazole (TCBZR/ABZR) and the Rubino isolate from Uruguay, resistant to ABZ (TCBZS/ABZR), and compare them to a sensitive strain (Cenapa, Mexico, TCBZS/ABZS) to reveal putative molecular mechanisms leading to drug resistance. We observed a major reduction in transcription in the Cajamarca TCBZR/ABZR isolate in comparison to the other isolates. While most of the differentially expressed genes are still unannotated, several trends could be detected. Specific reduction in the expression levels of cytoskeleton proteins was consistent with a role of tubulins as putative targets of triclabendazole (TCBZ). A marked reduction of adenylate cyclase might be underlying pleiotropic effects on diverse metabolic pathways of the parasite. Upregulation of GST mu isoforms suggests this detoxifying mechanism as one of the strategies associated with resistance. Our results stress the value of transcriptomic approaches as a means of providing novel insights to advance the understanding of drug mode of action and drug resistance. The results provide evidence for pleiotropic variations in drug-resistant isolates consistent with early observations of TCBZ and ABZ effects and recent proteomic findings.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 50 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 16%
Student > Master 8 16%
Professor > Associate Professor 6 12%
Student > Ph. D. Student 5 10%
Student > Bachelor 4 8%
Other 11 22%
Unknown 8 16%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 12 24%
Agricultural and Biological Sciences 10 20%
Biochemistry, Genetics and Molecular Biology 9 18%
Social Sciences 3 6%
Immunology and Microbiology 2 4%
Other 4 8%
Unknown 10 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2019.
All research outputs
#5,823,308
of 21,749,011 outputs
Outputs from Parasites & Vectors
#1,254
of 5,260 outputs
Outputs of similar age
#119,379
of 402,178 outputs
Outputs of similar age from Parasites & Vectors
#1
of 1 outputs
Altmetric has tracked 21,749,011 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 5,260 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 402,178 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them