Title |
MiR-203 down-regulates Rap1A and suppresses cell proliferation, adhesion and invasion in prostate cancer
|
---|---|
Published in |
Journal of Experimental & Clinical Cancer Research, January 2015
|
DOI | 10.1186/s13046-015-0125-x |
Pubmed ID | |
Authors |
Jun Xiang, Cuidong Bian, Hao Wang, Shengsong Huang, Denglong Wu |
Abstract |
ObjectiveEvidence supports an important role for miR-203 in the regulation of the proliferation, migration and invasion of prostate cancer (PCa) cells. However, the exact mechanisms of miR-203 in PCa are not entirely clear.MethodsWe examined the expression of miR-203 in prostate cancer tissues, adjacent normal tissues, PCa cell lines and normal prostate epithelial cells by qRT-PCR. Then, the effects of miR-203 or Rap1A on proliferation, adhesion and invasion of PCa cells were assayed using CKK-8, adhesion analysis, and transwell invasion assays. Luciferase reporter assay was performed to assess miR-203 binding to Rap1A mRNA. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.ResultsHere, we confirmed that the expression of miR-203 was significantly downregulated in prostate cancer specimens compared with matched adjacent normal prostate specimens. Mechanistic dissection revealed that miR-203 mediated cell proliferation, adhesion and invasion in vitro, and tumor growth in vivo, as evidenced by reduced RAC1, p-PAK1, and p-MEK1 expression. In addition, we identified Rap1A as a direct target suppressed by miR-203, and there was an inverse relationship between the expression of miR-203 and Rap1A in PCa. Knockdown of Rap1A phenocopied the effects of miR-203 on PCa cell growth and invasion. Furthermore, Rap1A over-expression in PCa cells partially reversed the effects of miR-203-expression on cell adhesion and invasion.ConclusionsThese findings provide further evidence that a crucial role for miR-203 in inhibiting metastasis of PCa through the suppression of Rap1A expression. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 1 | 6% |
Unknown | 15 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 25% |
Researcher | 3 | 19% |
Other | 1 | 6% |
Student > Master | 1 | 6% |
Student > Ph. D. Student | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 19% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Agricultural and Biological Sciences | 1 | 6% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Computer Science | 1 | 6% |
Other | 1 | 6% |
Unknown | 7 | 44% |