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MiR-130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells

Overview of attention for article published in BMC Cancer, January 2015
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Title
MiR-130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells
Published in
BMC Cancer, January 2015
DOI 10.1186/s12885-015-1031-5
Pubmed ID
Authors

Tingting Yu, Risheng Cao, Shuo Li, Mingen Fu, Lihua Ren, Weixu Chen, Hong Zhu, Qiang Zhan, Ruihua Shi

Abstract

BackgroundEsophageal carcinoma is one of the most common malignancies with high cancer-related morbidity and mortality worldwide. MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate a wide variety of cellular processes, and also play an important role in the development and progression of cancers. In a previous microarray study, we demonstrated that miR-130b was upregulated in esophageal squamous cell carcinoma (ESCC) tissues. However, the biologic functions and the molecular mechanism of miR-130b in ESCC remain to be elucidated.MethodsqRT-PCR assays were used to quantify miR-130b expression levels in ESCC samples. Novel targets of miR-130b were identified via a bioinformatics search and confirmed using a dual-luciferase reporter system. Western blotting and qRT-PCR assays were used to quantify the expression of the target gene PTEN (phosphatase and tensin homolog) and the downstream effector, Akt. ESCC cells over- or underexpressing miR-130b were analyzed for in vitro biologic functions.ResultsHigh levels of miR-130b were identified in 20 ESCC samples following comparison with adjacent non-neoplastic tissues. We confirmed that miR-130b interacted with the 3¿-untranslated region of PTEN, and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN. However, the dysregulation of miR-130b had no obvious impact on PTEN mRNA. As Akt is a downstream effector of PTEN, we explored if miR-130b affected Akt expression, and found that miR-130b indirectly regulated the level of phosphorylated Akt, while total Akt protein remained unchanged. Overexpression of miR-130b increased the proliferation of ESCC cells and enhanced their ability to migrate and invade. In contrast, the proliferation, migration, and invasion of ESCC cells were weakened when miR-130b expression was suppressed, which was reversed by PTEN-targeted siRNA.ConclusionThe results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation, which would be helpful in developing miRNA-based treatments for ESCC.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 4%
Unknown 24 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 32%
Student > Doctoral Student 4 16%
Researcher 3 12%
Student > Bachelor 2 8%
Student > Master 2 8%
Other 2 8%
Unknown 4 16%
Readers by discipline Count As %
Medicine and Dentistry 7 28%
Biochemistry, Genetics and Molecular Biology 6 24%
Agricultural and Biological Sciences 4 16%
Engineering 3 12%
Unknown 5 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 February 2015.
All research outputs
#3,169,135
of 4,733,346 outputs
Outputs from BMC Cancer
#1,456
of 2,633 outputs
Outputs of similar age
#107,924
of 165,760 outputs
Outputs of similar age from BMC Cancer
#54
of 76 outputs
Altmetric has tracked 4,733,346 research outputs across all sources so far. This one is in the 29th percentile – i.e., 29% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,633 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
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We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.