Title |
Kourami: graph-guided assembly for novel human leukocyte antigen allele discovery
|
---|---|
Published in |
Genome Biology, February 2018
|
DOI | 10.1186/s13059-018-1388-2 |
Pubmed ID | |
Authors |
Heewook Lee, Carl Kingsford |
Abstract |
Accurate typing of human leukocyte antigen (HLA) is important because HLA genes play important roles in immune responses and disease genesis. Previously available computational methods are database-matching approaches and their outputs are inherently limited by the completeness of already known types, making them unsuitable for discovery of novel alleles. We have developed a graph-guided assembly technique for classical HLA genes, which can construct allele sequences given high-coverage whole-genome sequencing data. Our method delivers highly accurate HLA typing, comparable to the current state-of-the-art methods. Using various data, we also demonstrate that our method can type novel alleles. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 7 | 44% |
United Kingdom | 3 | 19% |
India | 1 | 6% |
France | 1 | 6% |
Unknown | 4 | 25% |
Demographic breakdown
Type | Count | As % |
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Scientists | 8 | 50% |
Members of the public | 7 | 44% |
Science communicators (journalists, bloggers, editors) | 1 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 94 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 17 | 18% |
Researcher | 17 | 18% |
Student > Master | 9 | 10% |
Student > Bachelor | 9 | 10% |
Other | 6 | 6% |
Other | 15 | 16% |
Unknown | 21 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 27 | 29% |
Agricultural and Biological Sciences | 17 | 18% |
Computer Science | 13 | 14% |
Medicine and Dentistry | 5 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
Other | 6 | 6% |
Unknown | 25 | 27% |