Title |
Direct serogrouping of Dichelobacter nodosus from Victorian farms using conventional multiplex polymerase chain reaction
|
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Published in |
BMC Research Notes, February 2018
|
DOI | 10.1186/s13104-018-3229-5 |
Pubmed ID | |
Authors |
Nickala Best, Jacek Gwozdz, Robert Suter, Grant Rawlin, Travis Beddoe |
Abstract |
Dichelobacter nodosus is the causative agent of footrot in sheep. Ovine footrot is a major problem in Australia that results in large economic losses and a represents a very significant animal welfare issue. D. nodosus is divided into 10 serogroups (A-I, M), based on sequence variation in the type IV fimbriae gene, fimA. Control of the bacteria is possible through use of serogroup-specific vaccination, however traditional identification of the serogroups of D. nodosus on infected sheep is time-consuming and costly. With the aim of reducing time and cost, a PCR assay was used to identify serogroups of D. nodosus directly from foot swabs of infected sheep in Victoria. It was shown that serogroup B was most common (10 locations), followed by A, G and H (4 locations), I and C (2 locations), D, E and F (1 location). Infections with multiple serotypes were observed in 50% of farms, with the remaining 50% having only a single serogroup detected. The ability to identify serogroups quickly and cheaply direct from foot swabs will aid the understanding of the epidemiology of D. nodosus and support control programs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 25% |
Student > Bachelor | 2 | 17% |
Student > Master | 2 | 17% |
Professor | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 6 | 50% |
Agricultural and Biological Sciences | 2 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Social Sciences | 1 | 8% |
Unknown | 2 | 17% |