Title |
Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
|
---|---|
Published in |
Journal of Nanobiotechnology, May 2015
|
DOI | 10.1186/s12951-015-0096-2 |
Pubmed ID | |
Authors |
Jian-feng Pan, Ning-hua Liu, Lin-yuan Shu, Hui Sun |
Abstract |
Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time. PLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously. PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Romania | 1 | 3% |
Unknown | 37 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 18% |
Student > Master | 7 | 18% |
Student > Ph. D. Student | 6 | 16% |
Student > Bachelor | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Other | 4 | 11% |
Unknown | 7 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 16% |
Engineering | 6 | 16% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Chemistry | 3 | 8% |
Psychology | 2 | 5% |
Other | 10 | 26% |
Unknown | 7 | 18% |