Title |
Sequence analysis of percent G+C fraction libraries of human faecal bacterial DNA reveals a high number of Actinobacteria
|
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Published in |
BMC Microbiology, April 2009
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DOI | 10.1186/1471-2180-9-68 |
Pubmed ID | |
Authors |
Lotta Krogius-Kurikka, Anna Kassinen, Lars Paulin, Jukka Corander, Harri Mäkivuokko, Jarno Tuimala, Airi Palva |
Abstract |
The human gastrointestinal (GI) tract microbiota is characterised by an abundance of uncultured bacteria most often assigned in phyla Firmicutes and Bacteroidetes. Diversity of this microbiota, even though approached with culture independent techniques in several studies, still requires more elucidation. The main purpose of this work was to study whether the genomic percent guanine and cytosine (%G+C) -based profiling and fractioning prior to 16S rRNA gene sequence analysis reveal higher microbiota diversity, especially with high G+C bacteria suggested to be underrepresented in previous studies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 2% |
Sweden | 1 | 2% |
France | 1 | 2% |
Belgium | 1 | 2% |
Unknown | 42 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 28% |
Student > Ph. D. Student | 11 | 24% |
Professor | 4 | 9% |
Student > Bachelor | 3 | 7% |
Professor > Associate Professor | 3 | 7% |
Other | 6 | 13% |
Unknown | 6 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 20 | 43% |
Medicine and Dentistry | 6 | 13% |
Biochemistry, Genetics and Molecular Biology | 4 | 9% |
Veterinary Science and Veterinary Medicine | 2 | 4% |
Immunology and Microbiology | 2 | 4% |
Other | 5 | 11% |
Unknown | 7 | 15% |