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Mendeley readers
| Title |
Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
|
|---|---|
| Published in |
BMC Research Notes, February 2018
|
| DOI | 10.1186/s13104-018-3223-y |
| Pubmed ID | |
| Authors |
Abd Al-Bar Al-Farha, Kiro Petrovski, Razi Jozani, Andrew Hoare, Farhid Hemmatzadeh |
| Abstract |
This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia. One set of genus-level universal primers was designed targeting the 16S ribosomal RNA gene. Real time PCR-HRM analysis was able to identify and distinguish between five different mollicutes, namely A. laidlawii, M. arginini, M. bovirhinis, M. bovis and uncultured Mycoplasma. Results were confirmed through sequencing. Our developed assay provides rapid and accurate screening for Mycoplasma mastitis detection. |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 21% |
| Student > Ph. D. Student | 5 | 21% |
| Other | 2 | 8% |
| Student > Bachelor | 1 | 4% |
| Professor | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 10 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 5 | 21% |
| Agricultural and Biological Sciences | 5 | 21% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Medicine and Dentistry | 1 | 4% |
| Engineering | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 10 | 42% |