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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Purification and characterization of a novel defensin from the salivary glands of the black fly, Simulium bannaense
|
|---|---|
| Published in |
Parasites & Vectors, February 2015
|
| DOI | 10.1186/s13071-015-0669-9 |
| Pubmed ID | |
| Authors |
Lin Wei, Lixian Mu, Yipeng Wang, Hui Bian, Jun Li, Yiling Lu, Yi Han, Tong Liu, Jing Lv, Cuiping Feng, Jing Wu, Hailong Yang |
| Abstract |
BackgroundBlack flies (Diptera: Simuliidae) are haematophagous insects that can cause allergic reactions and act as vectors of pathogens. Although their saliva has been thought to contain a diverse array of physiologically active molecules, little information is available on antimicrobial factors in black fly salivary glands, especially no defensins have been reported so far.MethodsA novel cationic defensin designated SibaDef was purified using reverse phase high-performance liquid chromatography (RP-HPLC) from the salivary glands of the black fly Simulium bannaense. The amino acid sequence of SibaDef was determined by a combination method of automated Edman degradation and cDNA sequencing. The morphologic changes of Gram-positive bacteria Staphylococcus aureus or Bacillus subtilis treated with SibaDef were assessed by scanning electron microscopy (SEM). Quantitative PCR (qPCR) was performed to analyze the expression of SibaDef mRNA in whole bodies of insects after oral infection with the bacteria S. aureus or B. subtilis.ResultsSurprisingly, the phylogenetic analysis of defensin-related amino acid sequences demonstrated that SibaDef is most closely related to defensins from the human body louse Pediculus humanus corporis (Anoplura: Pediculidae), rather than to other dipteran defensins. SibaDef showed potent antimicrobial activities against Gram-positive bacteria with minimal inhibitory concentrations (MICs) ranging from 0.83 ¿M to 2.29 ¿M. SEM analysis indicated that SibaDef killed microorganisms through the disruption of cell membrane integrity. The transcript levels of SibaDef in the bacteria-immunized flies increased with the time course, reaching maximum at 36 h and then slowly decreased from that time point.ConclusionsOur results indicate that SibaDef is involved in the innate humoral response of the black fly S. bannaense, and it might play a significant role in the defence against microorganisms in both sugar and blood meals. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Madagascar | 1 | 3% |
| Unknown | 36 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 19% |
| Researcher | 7 | 19% |
| Student > Ph. D. Student | 6 | 16% |
| Student > Bachelor | 4 | 11% |
| Lecturer > Senior Lecturer | 2 | 5% |
| Other | 4 | 11% |
| Unknown | 7 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 27% |
| Biochemistry, Genetics and Molecular Biology | 8 | 22% |
| Chemistry | 3 | 8% |
| Environmental Science | 2 | 5% |
| Immunology and Microbiology | 2 | 5% |
| Other | 4 | 11% |
| Unknown | 8 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2016.
All research outputs
#13,932,002
of 22,786,691 outputs
Outputs from Parasites & Vectors
#2,648
of 5,457 outputs
Outputs of similar age
#181,747
of 352,275 outputs
Outputs of similar age from Parasites & Vectors
#58
of 149 outputs
Altmetric has tracked 22,786,691 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,457 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,275 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 149 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.