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Estimating trematode prevalence in snail hosts using a single-step duplex PCR: how badly does cercarial shedding underestimate infection rates?

Overview of attention for article published in Parasites & Vectors, May 2014
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Title
Estimating trematode prevalence in snail hosts using a single-step duplex PCR: how badly does cercarial shedding underestimate infection rates?
Published in
Parasites & Vectors, May 2014
DOI 10.1186/1756-3305-7-243
Pubmed ID
Authors

Ana Born-Torrijos, Robert Poulin, Juan Antonio Raga, Astrid Sibylle Holzer

Abstract

Trematode communities often consist of different species exploiting the same host population, with two or more trematodes sometimes co-occuring in the same host. A commonly used diagnostic method to detect larval trematode infections in snails has been based on cercarial shedding, though it is often criticized as inaccurate. In the present study we compare infection prevalences determined by cercarial emission with those determined, for the first time, by molecular methods, allowing us to quantify the underestimation of single and double infections based on cercarial emission. We thus developed a duplex PCR for two host-parasite systems, to specifically differentiate between single and double infections. The Ebro samples include two morphologically similar opecoelids, whereas the Otago samples include two morphologically different larval trematodes.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 3%
Spain 1 1%
Austria 1 1%
Unknown 83 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 20%
Student > Master 15 17%
Student > Bachelor 13 15%
Researcher 11 13%
Professor 4 5%
Other 15 17%
Unknown 12 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 38%
Environmental Science 13 15%
Biochemistry, Genetics and Molecular Biology 9 10%
Veterinary Science and Veterinary Medicine 6 7%
Medicine and Dentistry 6 7%
Other 9 10%
Unknown 12 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2015.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Parasites & Vectors
#5,345
of 5,986 outputs
Outputs of similar age
#208,288
of 241,408 outputs
Outputs of similar age from Parasites & Vectors
#89
of 104 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,986 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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