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Mendeley readers
Attention Score in Context
| Title |
Amplification of pico-scale DNA mediated by bacterial carrier DNA for small-cell-number transcription factor ChIP-seq
|
|---|---|
| Published in |
BMC Genomics, February 2015
|
| DOI | 10.1186/s12864-014-1195-4 |
| Pubmed ID | |
| Authors |
Janus S Jakobsen, Frederik O Bagger, Marie S Hasemann, Mikkel B Schuster, Anne-Katrine Frank, Johannes Waage, Kristoffer Vitting-Seerup, Bo T Porse |
| Abstract |
BackgroundChromatin-Immunoprecipitation coupled with deep sequencing (ChIP-seq) is used to map transcription factor occupancy and generate epigenetic profiles genome-wide. The requirement of nano-scale ChIP DNA for generation of sequencing libraries has impeded ChIP-seq on in vivo tissues of low cell numbers.ResultsWe describe a robust, simple and scalable methodology for ChIP-seq of low-abundant cell populations, verified down to 10,000 cells. By employing non-mammalian genome mapping bacterial carrier DNA during amplification, we reliably amplify down to 50 pg of ChIP DNA from transcription factor (CEBPA) and histone mark (H3K4me3) ChIP. We further demonstrate that genomic profiles are highly resilient to changes in carrier DNA to ChIP DNA ratios.ConclusionsThis represents a significant advance compared to existing technologies, which involve either complex steps of pre-selection for nucleosome-containing chromatin or pre-amplification of precipitated DNA, making them prone to introduce experimental biases. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 33% |
| United States | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 3% |
| United Kingdom | 1 | 1% |
| Denmark | 1 | 1% |
| Unknown | 67 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 20% |
| Researcher | 14 | 20% |
| Student > Master | 10 | 14% |
| Student > Bachelor | 7 | 10% |
| Professor > Associate Professor | 6 | 8% |
| Other | 14 | 20% |
| Unknown | 6 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 24 | 34% |
| Agricultural and Biological Sciences | 20 | 28% |
| Medicine and Dentistry | 6 | 8% |
| Engineering | 4 | 6% |
| Neuroscience | 3 | 4% |
| Other | 4 | 6% |
| Unknown | 10 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2016.
All research outputs
#6,413,521
of 22,786,691 outputs
Outputs from BMC Genomics
#2,879
of 10,647 outputs
Outputs of similar age
#89,012
of 352,181 outputs
Outputs of similar age from BMC Genomics
#75
of 248 outputs
Altmetric has tracked 22,786,691 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 10,647 research outputs from this source. They receive a mean Attention Score of 4.7. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,181 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 248 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.