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A laboratory test based on determination of cytokine profiles: a promising assay to identify exposition to contact allergens and predict the clinical outcome in occupational allergic contact…

Overview of attention for article published in BMC Immunology, February 2015
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About this Attention Score

  • Among the highest-scoring outputs from this source (#43 of 245)
  • Good Attention Score compared to outputs of the same age (70th percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Mentioned by

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5 tweeters

Citations

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7 Dimensions

Readers on

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28 Mendeley
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Title
A laboratory test based on determination of cytokine profiles: a promising assay to identify exposition to contact allergens and predict the clinical outcome in occupational allergic contact dermatitis
Published in
BMC Immunology, February 2015
DOI 10.1186/s12865-015-0066-3
Pubmed ID
Authors

Valentina Bordignon, Francesca Palamara, Giorgia Altomonte, Isabella Sperduti, Mario Pietravalle, Claudia Cavallotti, Paola Cordiali-Fei, Maria Pia Fuggetta, Antonio Cristaudo, Fabrizio Ensoli

Abstract

BackgroundPara-phenylenediamine (PPD) is the main allergen causing adverse reactions to hair dyes and a frequent cause of occupational-related skin sensitization among hairdressers and beauticians. The immunologic mechanism of the disease relies on the production of inflammatory cytokines by allergen-specific T cells, while regulatory T cells are thought to down-modulate the allergic response. This study was aimed at investigating the expression of effector or regulatory cytokines in exposed subjects in order to verify whether different cytokine profiles might predict distinct clinical outcomes. Peripheral blood mononuclear cells (PBMC) from 21 subjects occupationally exposed or not (10) to PPD were kept in short term cultures in the presence of optimized concentrations of NiSO4 x 6H2O or PPD. The production of IFN-¿ and IL-10 elicited by antigens were analyzed by the ELISpot assay.ResultsThe presence of IFN-¿ responses toward PPD was significantly correlated with a positive patch test (P¿=¿0.002) and allergic symptoms, while IL10 responses were invariably found in PPD-exposed but clinically asymptomatic subjects with negative patch testing. We found concordance between the different cytokine profiles and patch test results. No false-positive results were found for the different cytokine profiles induced by PPD, resulting in 100% specificity. The sensitivity of the test was 87.5% (95%CI 65.9-100.0) with an overall test accuracy of 93.3%. Although larger prospective-retrospective studies are necessary to validate the predictive potential of the test, the negative and positive predicted values for PPD in this study were NPV¿=¿87.5% and PPV¿=¿100%, respectively.ConclusionsThese data indicate that distinct cytokine profiles are associated with different clinical manifestations. The test, which is based on a simple and rapid profiling of cytokine responses by T lymphocytes against allergens, has proven to be a promising laboratory tool, useful for both the identification of previous contact with allergens and the etiologic diagnosis of contact allergies as well as capable of predicting the clinical outcome (development of an allergic or tolerant response).

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 4%
Belgium 1 4%
Brazil 1 4%
Unknown 25 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 21%
Student > Bachelor 4 14%
Student > Master 3 11%
Other 2 7%
Professor > Associate Professor 2 7%
Other 5 18%
Unknown 6 21%
Readers by discipline Count As %
Medicine and Dentistry 8 29%
Agricultural and Biological Sciences 3 11%
Biochemistry, Genetics and Molecular Biology 2 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Arts and Humanities 1 4%
Other 5 18%
Unknown 7 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2016.
All research outputs
#1,632,067
of 6,978,736 outputs
Outputs from BMC Immunology
#43
of 245 outputs
Outputs of similar age
#64,048
of 230,841 outputs
Outputs of similar age from BMC Immunology
#2
of 7 outputs
Altmetric has tracked 6,978,736 research outputs across all sources so far. This one has received more attention than most of these and is in the 65th percentile.
So far Altmetric has tracked 245 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 230,841 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.