Title |
Expression of Amphiphysin I, an Autoantigen of Paraneoplastic Neurological Syndromes, in Breast Cancer
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Published in |
Molecular Medicine, January 1998
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DOI | 10.1007/bf03401727 |
Pubmed ID | |
Authors |
Scott Floyd, Margaret Husta Butler, Ottavio Cremona, Carol David, Zachary Freyberg, Xiaomei Zhang, Michele Solimena, Akira Tokunaga, Hideki Ishizu, Kimiko Tsutsui, Pietro De Camilli |
Abstract |
Amphiphysin I is a 128 kD protein highly concentrated in nerve terminals, where it has a putative role in endocytosis. It is a dominant autoantigen in patients with stiff-man syndrome associated with breast cancer, as well as in other paraneoplastic autoimmune neurological disorders. To elucidate the connection between amphiphysin I autoimmunity and cancer, we investigated its expression in breast cancer tissue. We report that amphiphysin I was expressed as two isoforms of 128 and 108 kD in the breast cancer of a patient with anti-amphiphysin I antibodies and paraneoplastic sensory neuronopathy. Amphiphysin I was also detectable at variable levels in several other human breast cancer tissues and cell lines and at low levels in normal mammary tissue and a variety of other non-neuronal tissues. The predominant amphiphysin I isoform expressed outside the brain in humans is the 108 kD isoform which represents an alternatively spliced variant of neuronal amphiphysin I missing a 42 amino acid insert. Our study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells. |
Mendeley readers
Geographical breakdown
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France | 1 | 3% |
Unknown | 28 | 90% |
Demographic breakdown
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Professor > Associate Professor | 5 | 16% |
Student > Bachelor | 3 | 10% |
Professor | 3 | 10% |
Student > Doctoral Student | 3 | 10% |
Other | 8 | 26% |
Unknown | 4 | 13% |
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Chemistry | 2 | 6% |
Other | 2 | 6% |
Unknown | 2 | 6% |