Title |
Improved methods for magnetic purification of malaria parasites and haemozoin
|
---|---|
Published in |
Malaria Journal, January 2010
|
DOI | 10.1186/1475-2875-9-17 |
Pubmed ID | |
Authors |
Charles C Kim, Emily B Wilson, Joseph L DeRisi |
Abstract |
Malaria parasites generate free haem upon catabolism of host haemoglobin during their intraerythrocytic growth cycle. In order to minimize oxidative toxicity of the ferric iron, the free haem molecules are polymerized into the biomineral beta-haematin (commonly referred to as haemozoin). Haemozoin crystals are paramagnetic, and this property can be exploited for the purification of late stage parasites as they contain larger haemozoin crystals than early stage parasites and uninfected cells. Commercially available magnets that were originally developed for the purpose of antibody-mediated cell purification are widely used for this purpose. As these methods are not necessarily optimized for parasite purification, the relationship between magnetic field strength and the quantity and quality of yield during parasite purification was explored. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 3% |
Canada | 2 | 2% |
United Kingdom | 1 | <1% |
Brazil | 1 | <1% |
Unknown | 102 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 33 | 30% |
Student > Bachelor | 14 | 13% |
Researcher | 13 | 12% |
Student > Master | 12 | 11% |
Student > Doctoral Student | 7 | 6% |
Other | 16 | 15% |
Unknown | 14 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 40 | 37% |
Biochemistry, Genetics and Molecular Biology | 16 | 15% |
Engineering | 13 | 12% |
Physics and Astronomy | 8 | 7% |
Immunology and Microbiology | 7 | 6% |
Other | 9 | 8% |
Unknown | 16 | 15% |