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Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage

Overview of attention for article published in Parasites & Vectors, February 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (69th percentile)
  • Good Attention Score compared to outputs of the same age and source (67th percentile)

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3 tweeters
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1 Facebook page

Citations

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35 Dimensions

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18 Mendeley
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Title
Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage
Published in
Parasites & Vectors, February 2015
DOI 10.1186/s13071-015-0689-5
Pubmed ID
Authors

Bin Tang, Mingyuan Liu, Libo Wang, Shenye Yu, Haining Shi, Pascal Boireau, Vasile Cozma, Xiuping Wu, Xiaolei Liu

Abstract

BackgroundThe intestinal phase is the early invasion stage of Trichinella spiralis (T. spiralis), in which muscle larvae invade intestine epithelial cells and then develop into adult worms to breed newborn larvae. Thus, intestinal infective larvae are first exposed to the immune system of the host, and antigens from the worms may be the earliest marker in the diagnosis of trichinellosis and may contribute to vaccine development to prevent Trichinella infections in pigs.MethodsA cDNA library of intestinal infective larvae of T. spiralis at 6 hours post infection (p.i.) was constructed and immunoscreened using serum collected from pigs that were infected with T. spiralis at 26 days p.i. T. spiralis cystatin-like protein (Ts-CLP) gene encoding a 45.9 kDa protein was cloned and expressed in Escherichia coli. The rabbit antisera were generated and used to determine the location of Ts-CLP in the parasite. Transcription levels of Ts-CLP in different developmental stages of T. spiralis were observed by RT-PCR. The potential application of recombinant Ts-CLP in diagnosis against T. spiralis infection was tested by ELISA. The immune protection of recombinant Ts-CLP protein against T. spiralis infection was evaluated in mice.ResultsThirty-three positive clones were selected from cDNA library, among which 20 clones encoded the same novel cystatin-like protein (Ts-CLP). Immunolocalisation and real-time quantitative PCR revealed that native Ts-CLP was localised primarily to ß-stichocytes and that the Ts-clp gene was transcribed and expressed in all developmental stages of T. spiralis. The recombinant protein rTs-CLP was recognised by pig antiserum as early as 15 days p.i., and could induce protective immunity in mice, with a 61.21% reduction in the number of muscle larvae.ConclusionsThese data preliminarily suggested that Ts-CLP may play an important role in the early infection of T. spiralis and that recombinant Ts-CLP protein is a candidate antigen for diagnosis and vaccine development in Trichinella infections.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Romania 1 6%
Unknown 17 94%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 17%
Researcher 3 17%
Student > Master 3 17%
Professor 1 6%
Other 1 6%
Other 2 11%
Unknown 5 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 17%
Agricultural and Biological Sciences 3 17%
Medicine and Dentistry 2 11%
Immunology and Microbiology 2 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Other 3 17%
Unknown 4 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 January 2016.
All research outputs
#2,434,098
of 6,920,439 outputs
Outputs from Parasites & Vectors
#515
of 1,875 outputs
Outputs of similar age
#69,303
of 230,547 outputs
Outputs of similar age from Parasites & Vectors
#34
of 106 outputs
Altmetric has tracked 6,920,439 research outputs across all sources so far. This one has received more attention than most of these and is in the 64th percentile.
So far Altmetric has tracked 1,875 research outputs from this source. They receive a mean Attention Score of 3.6. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 230,547 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 106 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.