Title |
Laboratory assessment of sensitive molecular tools for detection of low levels of Echinococcus multilocularis-eggs in fox (Vulpes vulpes) faeces
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Published in |
Parasites & Vectors, May 2014
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DOI | 10.1186/1756-3305-7-246 |
Pubmed ID | |
Authors |
Øivind Øines, Mats Isaksson, Åsa Hagström, Saraya Tavornpanich, Rebecca K Davidson |
Abstract |
In endemic areas with very low infection prevalence, the frequency and intensity of Echinococcus multilocularis can be extremely low. This necessitates efficient, specific and sensitive molecular tools. We wanted to compare the existing molecular tools, used in the Norwegian national surveillance programme, and compare these with new techniques for detection of this zoonotic pathogen in fox faeces. Here we present the results of screening samples containing a known level of E. multilocularis eggs with two highly sensitive DNA isolation and extraction methods combined with one conventional PCR and three real-time PCR methods for detection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 3% |
Denmark | 1 | 3% |
Unknown | 32 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 11 | 32% |
Student > Master | 7 | 21% |
Student > Ph. D. Student | 5 | 15% |
Professor | 3 | 9% |
Student > Bachelor | 2 | 6% |
Other | 3 | 9% |
Unknown | 3 | 9% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 11 | 32% |
Veterinary Science and Veterinary Medicine | 8 | 24% |
Medicine and Dentistry | 3 | 9% |
Environmental Science | 2 | 6% |
Immunology and Microbiology | 2 | 6% |
Other | 2 | 6% |
Unknown | 6 | 18% |