Title |
Genome-wide mapping of ORC and Mcm2p binding sites on tiling arrays and identification of essential ARS consensus sequences in S. cerevisiae
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Published in |
BMC Genomics, October 2006
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DOI | 10.1186/1471-2164-7-276 |
Pubmed ID | |
Authors |
Weihong Xu, Jennifer G Aparicio, Oscar M Aparicio, Simon Tavaré |
Abstract |
Eukaryotic replication origins exhibit different initiation efficiencies and activation times within S-phase. Although local chromatin structure and function influences origin activity, the exact mechanisms remain poorly understood. A key to understanding the exact features of chromatin that impinge on replication origin function is to define the precise locations of the DNA sequences that control origin function. In S. cerevisiae, Autonomously Replicating Sequences (ARSs) contain a consensus sequence (ACS) that binds the Origin Recognition Complex (ORC) and is essential for origin function. However, an ACS is not sufficient for origin function and the majority of ACS matches do not function as ORC binding sites, complicating the specific identification of these sites. |
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Geographical breakdown
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Germany | 3 | 3% |
United Kingdom | 2 | 2% |
Spain | 2 | 2% |
France | 1 | <1% |
Estonia | 1 | <1% |
Unknown | 95 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 32 | 30% |
Researcher | 26 | 24% |
Student > Master | 18 | 17% |
Student > Doctoral Student | 6 | 6% |
Professor > Associate Professor | 6 | 6% |
Other | 10 | 9% |
Unknown | 10 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 59 | 55% |
Biochemistry, Genetics and Molecular Biology | 32 | 30% |
Physics and Astronomy | 2 | 2% |
Chemistry | 2 | 2% |
Immunology and Microbiology | 1 | <1% |
Other | 3 | 3% |
Unknown | 9 | 8% |