Title |
miR-190 suppresses breast cancer metastasis by regulation of TGF-β-induced epithelial–mesenchymal transition
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Published in |
Molecular Cancer, March 2018
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DOI | 10.1186/s12943-018-0818-9 |
Pubmed ID | |
Authors |
Yue Yu, Wei Luo, Zheng-Jun Yang, Jiang-Rui Chi, Yun-Rui Li, Yu Ding, Jie Ge, Xin Wang, Xu-Chen Cao |
Abstract |
Breast cancer is the most common cancer among women worldwide and metastasis is the leading cause of death among patients with breast cancer. The transforming growth factor-β (TGF-β) pathway plays critical roles during breast cancer epithelial-mesenchymal transition (EMT) and metastasis. SMAD2, a positive regulator of TGF-β signaling, promotes breast cancer metastasis through induction of EMT. The expression of miR-190 and SMAD2 in breast cancer tissues, adjacent normal breast tissues and cell lines were determined by RT-qPCR. The protein expression levels and localization were analyzed by western blotting and immunofluorescence. ChIP and dual-luciferase report assays were used to validate the regulation of ZEB1-miR-190-SMAD2 axis. The effect of miR-190 on breast cancer progression was investigated both in vitro and in vivo. miR-190 down-regulation is required for TGF-β-induced EMT. miR-190 suppresses breast cancer metastasis both in vitro and in vivo by targeting SMAD2. miR-190 expression is down-regulated and inversely correlates with SMAD2 in breast cancer samples, and its expression level was associated with outcome in patients with breast cancer. Furthermore, miR-190 is transcriptionally regulated by ZEB1. Our data uncover the ZEB1-miR-190-SMAD2 axis and provide a mechanism to explain the TGF-β network in breast cancer metastasis. |
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Unknown | 1 | 100% |
Demographic breakdown
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 50 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 9 | 18% |
Student > Ph. D. Student | 7 | 14% |
Student > Postgraduate | 5 | 10% |
Researcher | 5 | 10% |
Student > Bachelor | 4 | 8% |
Other | 3 | 6% |
Unknown | 17 | 34% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 21 | 42% |
Agricultural and Biological Sciences | 4 | 8% |
Medicine and Dentistry | 2 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Unspecified | 1 | 2% |
Other | 4 | 8% |
Unknown | 17 | 34% |