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Protection induced by a gp90 protein-based vaccine derived from a Reticuloendotheliosis virus strain isolated from a contaminated IBD vaccine

Overview of attention for article published in Virology Journal, March 2018
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Title
Protection induced by a gp90 protein-based vaccine derived from a Reticuloendotheliosis virus strain isolated from a contaminated IBD vaccine
Published in
Virology Journal, March 2018
DOI 10.1186/s12985-018-0948-0
Pubmed ID
Authors

Zhihao Ren, Fanfeng Meng, Qiuchen Li, Yixin Wang, Xiaofeng Liu, Zhizhong Cui, Shuang Chang, Peng Zhao

Abstract

Reticuloendotheliosis is an immunosuppressive disease caused by avian reticuloendotheliosis virus (REV). It is commonly found in poultry farms and has caused a notable economic loss worldwide. Despite this, there is currently no effective vaccine available to protect against REV infection. In this study, gp90 protein derived from an REV isolated from a contaminated vaccine was co-administered with cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) adjuvant to hens to determine if it protects their chicks against REV infection. To synthesize the gp90 protein, the gp90 gene was amplified using polymerase chain reaction, expressed in Escherichia coli, and purified. The resulting recombinant protein was injected intramuscularly into breeder hens along with CpG-ODN adjuvant and then serum antibody levels were regularly evaluated. After the fertilized eggs from these vaccinated hens had hatched, the resulting chicks were challenged with a 102.7 50% tissue culture infectious dose (TCID50) of REV at 1 day old and the REV antibody levels in these hatched chickens were evaluated before and after the challenge. Viremia and growth rate were measured weekly and statistically analyzed. The results suggest that the gp90 recombinant protein was successfully prepared and, when used with CpG-ODN adjuvant to immunize breeder hens, induced serological antibody production against REV in both hens and their hatched chicks. In addition, the maternal antibodies induced by the gp90 protein vaccine effectively protected majority of the chicks from REV infection. Overall, we found the gp90 protein obtained in this study may be a potential vaccine candidate that had good immunogenicity and could be an auxiliary measure to accelerate the eradication of REV.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 30%
Student > Ph. D. Student 2 20%
Student > Postgraduate 2 20%
Unspecified 1 10%
Researcher 1 10%
Other 0 0%
Unknown 1 10%
Readers by discipline Count As %
Medicine and Dentistry 3 30%
Veterinary Science and Veterinary Medicine 2 20%
Nursing and Health Professions 1 10%
Unspecified 1 10%
Social Sciences 1 10%
Other 1 10%
Unknown 1 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2018.
All research outputs
#10,110,275
of 12,639,455 outputs
Outputs from Virology Journal
#1,662
of 2,112 outputs
Outputs of similar age
#205,812
of 274,154 outputs
Outputs of similar age from Virology Journal
#1
of 1 outputs
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So far Altmetric has tracked 2,112 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
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