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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality
|
|---|---|
| Published in |
Lipids in Health and Disease, March 2018
|
| DOI | 10.1186/s12944-018-0695-7 |
| Pubmed ID | |
| Authors |
Mónica Muñoz-Vega, Felipe Massó, Araceli Páez, Elizabeth Carreón-Torres, Hector A. Cabrera-Fuentes, José Manuel Fragoso, Nonanzit Pérez-Hernández, Laurent O. Martinez, Souad Najib, Gilberto Vargas-Alarcón, Óscar Pérez-Méndez |
| Abstract |
Primary cultures endothelial cells have been used as models of endothelial related diseases such atherosclerosis. Biological behavior of primary cultures is donor-dependent and data could not be easily reproducible; endothelial cell lines are emerging options, particularly, human dermal microvascular endothelial cells (HMEC-1), that should be validated to substitute primary cultures for the study of HDL functions. Morphology, size and granularity of cells were assessed by phase contrast microscopy and flow cytometry of HMEC-1. The adhesion molecules, ICAM-1and VCAM-1 after TNF-α stimulation, and endothelial markers CD105 endoglin, as well as HDL receptor SR-BI were determined by flow cytometry. Internalization of HDL protein was demonstrated by confocal microscopy using HDL labeled with Alexa Fluor 488. HUVECs were used as reference to compared the characteristics with HMEC-1. HMEC-1 and HUVEC had similar morphologies, size and granularity. HMEC-1 expressed endothelial markers as HUVECs, as well as functional SR-B1 receptor since the cell line was able to internalize HDL particles. HMEC-1 effectively increased ICAM-1 and VCAM-1 expression after TNF-α stimulation. HUVECs showed more sensibility to TNF-α stimulus but the range of ICAM-1 and VCAM-1 expression was less homogeneous than in HMEC-1, probably due to biological variation of the former. Finally, the expression of adhesion molecules in HMEC-1 was attenuated by co-incubation with HDL. HMEC-1 possess characteristics of endothelial cells, similar to HUVECs, being a cell line suitable to evaluate the functionality of HDL vis-à-vis the endothelium. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 69 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 16% |
| Student > Bachelor | 10 | 14% |
| Student > Ph. D. Student | 7 | 10% |
| Student > Doctoral Student | 6 | 9% |
| Researcher | 5 | 7% |
| Other | 9 | 13% |
| Unknown | 21 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 18 | 26% |
| Immunology and Microbiology | 4 | 6% |
| Agricultural and Biological Sciences | 4 | 6% |
| Medicine and Dentistry | 3 | 4% |
| Chemistry | 3 | 4% |
| Other | 7 | 10% |
| Unknown | 30 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 March 2018.
All research outputs
#15,495,840
of 23,028,364 outputs
Outputs from Lipids in Health and Disease
#803
of 1,458 outputs
Outputs of similar age
#212,391
of 332,340 outputs
Outputs of similar age from Lipids in Health and Disease
#17
of 37 outputs
Altmetric has tracked 23,028,364 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,458 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.1. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 332,340 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.