The complete chloroplast genome sequence of Citrus sinensis(L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms

Michael G Bausher, Nameirakpam D Singh, Seung-Bum Lee, Robert K Jansen, Henry Daniell

The production of Citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease Citrus canker. No significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. Chloroplast genetic engineering has a number of advantages over nuclear transformation; it not only increases transgene expression but also facilitates transgene containment, which is one of the major impediments for development of transgenic trees. We have sequenced the Citrus chloroplast genome to facilitate genetic improvement of this crop and to assess phylogenetic relationships among major lineages of angiosperms.