Title |
Chronic exposure to tumor necrosis factor alpha induces retinal pigment epithelium cell dedifferentiation
|
---|---|
Published in |
Journal of Neuroinflammation, March 2018
|
DOI | 10.1186/s12974-018-1106-8 |
Pubmed ID | |
Authors |
Sara Touhami, Fanny Beguier, Sébastien Augustin, Hugo Charles-Messance, Lucile Vignaud, Emeline F. Nandrot, Sacha Reichman, Valérie Forster, Thibaud Mathis, José-Alain Sahel, Bahram Bodaghi, Xavier Guillonneau, Florian Sennlaub |
Abstract |
The retinal pigment epithelium (RPE) is a monolayer of pigmented cells with important barrier and immuno-suppressive functions in the eye. We have previously shown that acute stimulation of RPE cells by tumor necrosis factor alpha (TNFα) downregulates the expression of OTX2 (Orthodenticle homeobox 2) and dependent RPE genes. We here investigated the long-term effects of TNFα on RPE cell morphology and key functions in vitro. Primary porcine RPE cells were exposed to TNFα (at 0.8, 4, or 20 ng/ml per day) for 10 days. RPE cell morphology, phagocytosis, barrier- and immunosuppressive-functions were assessed. Chronic (10 days) exposure of primary RPE cells to TNFα increases RPE cell size and polynucleation, decreases visual cycle gene expression, impedes RPE tight-junction organization and transepithelial resistance, and decreases the immunosuppressive capacities of the RPE. TNFα-induced morphological- and transepithelial-resistance changes were prevented by concomitant Transforming Growth Factor β inhibition. Our results indicate that chronic TNFα-exposure is sufficient to alter RPE morphology and impede cardinal features that define the differentiated state of RPE cells with striking similarities to the alterations that are observed with age in neurodegenerative diseases such as age-related macular degeneration. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 41 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 24% |
Researcher | 7 | 17% |
Student > Bachelor | 6 | 15% |
Other | 4 | 10% |
Student > Postgraduate | 2 | 5% |
Other | 2 | 5% |
Unknown | 10 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 11 | 27% |
Agricultural and Biological Sciences | 5 | 12% |
Medicine and Dentistry | 3 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
Neuroscience | 2 | 5% |
Other | 5 | 12% |
Unknown | 13 | 32% |