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Mendeley readers
| Title |
A rapid and inexpensive labeling method for microarray gene expression analysis
|
|---|---|
| Published in |
BMC Biotechnology, November 2009
|
| DOI | 10.1186/1472-6750-9-97 |
| Pubmed ID | |
| Authors |
Mario Ouellet, Paul D Adams, Jay D Keasling, Aindrila Mukhopadhyay |
| Abstract |
Global gene expression profiling by DNA microarrays is an invaluable tool in biological research. However, existing labeling methods are time consuming and costly and therefore often limit the scale of microarray experiments and sample throughput. Here we introduce a new, fast, inexpensive method for direct random-primed fluorescent labeling of eukaryotic cDNA for gene expression analysis and compare the results obtained on the NimbleGen microarray platform with two other widely-used labeling methods, namely the NimbleGen-recommended double-stranded cDNA protocol and the indirect (aminoallyl) method. |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 4% |
| Switzerland | 1 | 2% |
| Portugal | 1 | 2% |
| Mexico | 1 | 2% |
| Brazil | 1 | 2% |
| Unknown | 39 | 87% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 22 | 49% |
| Student > Ph. D. Student | 7 | 16% |
| Student > Bachelor | 3 | 7% |
| Professor | 3 | 7% |
| Student > Postgraduate | 3 | 7% |
| Other | 5 | 11% |
| Unknown | 2 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 53% |
| Biochemistry, Genetics and Molecular Biology | 6 | 13% |
| Medicine and Dentistry | 3 | 7% |
| Computer Science | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 5 | 11% |
| Unknown | 3 | 7% |