Title |
Norvaline is accumulated after a down-shift of oxygen in Escherichia coli W3110
|
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Published in |
Microbial Cell Factories, October 2008
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DOI | 10.1186/1475-2859-7-30 |
Pubmed ID | |
Authors |
Jaakko Soini, Christina Falschlehner, Christina Liedert, Jörg Bernhardt, Jussi Vuoristo, Peter Neubauer |
Abstract |
Norvaline is an unusual non-proteinogenic branched-chain amino acid which has been of interest especially during the early enzymological studies on regulatory mutants of the branched-chain amino acid pathway in Serratia marcescens. Only recently norvaline and other modified amino acids of the branched-chain amino acid synthesis pathway got attention again when they were found to be incorporated in minor amounts in heterologous proteins with a high leucine or methionine content. Earlier experiments have convincingly shown that norvaline and norleucine are formed from pyruvate being an alternative substrate of alpha-isopropylmalate synthase, however so far norvaline accumulation was not shown to occur in non-recombinant strains of E. coli. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | <1% |
United States | 1 | <1% |
Estonia | 1 | <1% |
Canada | 1 | <1% |
Unknown | 100 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 22 | 21% |
Student > Ph. D. Student | 18 | 17% |
Student > Master | 14 | 13% |
Student > Bachelor | 12 | 12% |
Other | 7 | 7% |
Other | 14 | 13% |
Unknown | 17 | 16% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 39 | 38% |
Biochemistry, Genetics and Molecular Biology | 19 | 18% |
Chemistry | 7 | 7% |
Engineering | 6 | 6% |
Environmental Science | 3 | 3% |
Other | 9 | 9% |
Unknown | 21 | 20% |