Title |
Fosmid library end sequencing reveals a rarely known genome structure of marine shrimp Penaeus monodon
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Published in |
BMC Genomics, May 2011
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DOI | 10.1186/1471-2164-12-242 |
Pubmed ID | |
Authors |
Shiao-Wei Huang, You-Yu Lin, En-Min You, Tze-Tze Liu, Hung-Yu Shu, Keh-Ming Wu, Shih-Feng Tsai, Chu-Fang Lo, Guang-Hsiung Kou, Gwo-Chin Ma, Ming Chen, Dongying Wu, Takashi Aoki, Ikuo Hirono, Hon-Tsen Yu |
Abstract |
The black tiger shrimp (Penaeus monodon) is one of the most important aquaculture species in the world, representing the crustacean lineage which possesses the greatest species diversity among marine invertebrates. Yet, we barely know anything about their genomic structure. To understand the organization and evolution of the P. monodon genome, a fosmid library consisting of 288,000 colonies and was constructed, equivalent to 5.3-fold coverage of the 2.17 Gb genome. Approximately 11.1 Mb of fosmid end sequences (FESs) from 20,926 non-redundant reads representing 0.45% of the P. monodon genome were obtained for repetitive and protein-coding sequence analyses. |
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Geographical breakdown
Country | Count | As % |
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Canada | 2 | 4% |
Norway | 1 | 2% |
Indonesia | 1 | 2% |
Sweden | 1 | 2% |
Brazil | 1 | 2% |
Unknown | 50 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 23% |
Student > Master | 11 | 20% |
Student > Ph. D. Student | 8 | 14% |
Professor | 4 | 7% |
Other | 3 | 5% |
Other | 7 | 13% |
Unknown | 10 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 31 | 55% |
Biochemistry, Genetics and Molecular Biology | 8 | 14% |
Medicine and Dentistry | 2 | 4% |
Environmental Science | 1 | 2% |
Immunology and Microbiology | 1 | 2% |
Other | 1 | 2% |
Unknown | 12 | 21% |