Title |
Reduced polyphenol oxidase gene expression and enzymatic browning in potato (Solanum tuberosum L.) with artificial microRNAs
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Published in |
BMC Plant Biology, March 2014
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DOI | 10.1186/1471-2229-14-62 |
Pubmed ID | |
Authors |
Ming Chi, Basdeo Bhagwat, W David Lane, Guiliang Tang, Yinquan Su, Runcang Sun, B Dave Oomah, Paul A Wiersma, Yu Xiang |
Abstract |
Polyphenol oxidase (PPO), often encoded by a multi-gene family, causes oxidative browning, a significant problem in many food products. Low-browning potatoes were produced previously through suppression of PPO gene expression, but the contribution of individual PPO gene isoform to the oxidative browning process was unknown. Here we investigated the contributions of different PPO genes to total PPO protein activity, and the correlations between PPO protein level, PPO activity and tuber tissue browning potential by suppression of all previously characterized potato PPO genes, both individually and in combination using artificial microRNAs (amiRNAs) technology. |
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