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Mendeley readers
Attention Score in Context
| Title |
The design and evaluation of a shaped filter collection device to sample and store defined volume dried blood spots from finger pricks
|
|---|---|
| Published in |
Malaria Journal, February 2015
|
| DOI | 10.1186/s12936-015-0558-x |
| Pubmed ID | |
| Authors |
Spencer D Polley, David Bell, James Oliver, Frank Tully, Mark D Perkins, Peter L Chiodini, Iveth J González |
| Abstract |
BackgroundDried blood spots are a common medium for collecting patient blood prior to testing for malaria by molecular methods. A new shaped filter device for the quick and simple collection of a designated volume of patient blood has been designed and tested against conventional blood spots for accuracy and precision.MethodsShaped filter devices were laser cut from Whatman GB003 paper to absorb a 20 ¿l blood volume. These devices were used to sample Plasmodium falciparum infected blood and the volume absorbed was measured volumetrically. Conventional blood spots were made by pipetting 20 ¿l of the same blood onto Whatman 3MM paper. DNA was extracted from both types of dried blood spot using Qiagen DNA blood mini or Chelex extraction for real-time PCR analysis, and PURE extraction for malaria LAMP testing.ResultsThe shaped filter devices collected a mean volume of 21.1 ¿l of blood, with a coefficient of variance of 8.1%. When used for DNA extraction by Chelex and Qiagen methodologies the mean number of international standard units of P. falciparum DNA recovered per ¿l of the eluate was 53.1 (95% CI: 49.4 to 56.7) and 32.7 (95% CI: 28.8 to 36.6), respectively for the shaped filter device, and 54.6 (95% CI: 52.1 to 57.1) and 12.0 (95% CI: 9.9 to 14.1), respectively for the 3MM blood spots. Qiagen extraction of 200 ¿l of whole infected blood yielded 853.6 international standard units of P. falciparum DNA per ¿l of eluate.ConclusionsA shaped filter device provides a simple way to quickly sample and store a defined volume of blood without the need for any additional measuring devices. Resultant dried blood spots may be employed for DNA extraction using a variety of technologies for nucleic acid amplification without the need for repeated cleaning of scissors or punches to prevent cross contamination of samples and results are comparable to traditional DBS. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Venezuela, Bolivarian Republic of | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Burkina Faso | 1 | 2% |
| Unknown | 54 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 25% |
| Researcher | 10 | 18% |
| Other | 5 | 9% |
| Student > Doctoral Student | 4 | 7% |
| Student > Bachelor | 3 | 5% |
| Other | 9 | 16% |
| Unknown | 10 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 16% |
| Medicine and Dentistry | 9 | 16% |
| Agricultural and Biological Sciences | 7 | 13% |
| Chemistry | 4 | 7% |
| Engineering | 4 | 7% |
| Other | 8 | 15% |
| Unknown | 14 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 February 2015.
All research outputs
#14,676,511
of 22,792,160 outputs
Outputs from Malaria Journal
#4,194
of 5,561 outputs
Outputs of similar age
#196,225
of 352,207 outputs
Outputs of similar age from Malaria Journal
#53
of 103 outputs
Altmetric has tracked 22,792,160 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,561 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,207 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 103 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.