Title |
Fast forward genetics to identify mutations causing a high light tolerant phenotype in Chlamydomonas reinhardtii by whole-genome-sequencing
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Published in |
BMC Genomics, February 2015
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DOI | 10.1186/s12864-015-1232-y |
Pubmed ID | |
Authors |
Lisa Schierenbeck, David Ries, Kristin Rogge, Sabrina Grewe, Bernd Weisshaar, Olaf Kruse |
Abstract |
High light tolerance of microalgae is a desired phenotype for efficient cultivation in large scale production systems under fluctuating outdoor conditions. Outdoor cultivation requires the use of either wild-type or non-GMO derived mutant strains due to safety concerns. The identification and molecular characterization of such mutants derived from untagged forward genetics approaches was limited previously by the tedious and time-consuming methods involving techniques such as classical meiotic mapping. The combination of mapping with next generation sequencing technologies offers alternative strategies to identify genes involved in high light adaptation in untagged mutants. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 2 | 2% |
United States | 1 | <1% |
Czechia | 1 | <1% |
Uruguay | 1 | <1% |
Unknown | 115 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 33 | 28% |
Researcher | 20 | 17% |
Student > Bachelor | 15 | 13% |
Student > Master | 10 | 8% |
Student > Doctoral Student | 7 | 6% |
Other | 14 | 12% |
Unknown | 21 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 49 | 41% |
Biochemistry, Genetics and Molecular Biology | 35 | 29% |
Computer Science | 3 | 3% |
Engineering | 3 | 3% |
Environmental Science | 1 | <1% |
Other | 4 | 3% |
Unknown | 25 | 21% |