Title |
Variations in genome-wide RNAi screens: lessons from influenza research
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Published in |
Journal of Clinical Bioinformatics, March 2015
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DOI | 10.1186/s13336-015-0017-5 |
Pubmed ID | |
Authors |
Yu-Chi Chou, Michael MC Lai, Yi-Chen Wu, Nai-Chi Hsu, King-Song Jeng, Wen-Chi Su |
Abstract |
Genome-wide RNA interference (RNAi) screening is an emerging and powerful technique for genetic screens, which can be divided into arrayed RNAi screen and pooled RNAi screen/selection based on different screening strategies. To date, several genome-wide RNAi screens have been successfully performed to identify host factors essential for influenza virus replication. However, the host factors identified by different research groups are not always consistent. Taking influenza virus screens as an example, we found that a number of screening parameters may directly or indirectly influence the primary hits identified by the screens. This review highlights the differences among the published genome-wide screening approaches and offers recommendations for performing a good pooled shRNA screen/selection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Turkey | 1 | 2% |
Austria | 1 | 2% |
United Kingdom | 1 | 2% |
Canada | 1 | 2% |
Denmark | 1 | 2% |
Unknown | 55 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 17 | 28% |
Researcher | 12 | 20% |
Student > Bachelor | 6 | 10% |
Student > Postgraduate | 5 | 8% |
Student > Master | 5 | 8% |
Other | 8 | 13% |
Unknown | 7 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 21 | 35% |
Biochemistry, Genetics and Molecular Biology | 17 | 28% |
Medicine and Dentistry | 5 | 8% |
Immunology and Microbiology | 4 | 7% |
Engineering | 2 | 3% |
Other | 3 | 5% |
Unknown | 8 | 13% |