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Variations in genome-wide RNAi screens: lessons from influenza research

Overview of attention for article published in Journal of Clinical Bioinformatics, March 2015
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Title
Variations in genome-wide RNAi screens: lessons from influenza research
Published in
Journal of Clinical Bioinformatics, March 2015
DOI 10.1186/s13336-015-0017-5
Pubmed ID
Authors

Yu-Chi Chou, Michael MC Lai, Yi-Chen Wu, Nai-Chi Hsu, King-Song Jeng, Wen-Chi Su

Abstract

Genome-wide RNA interference (RNAi) screening is an emerging and powerful technique for genetic screens, which can be divided into arrayed RNAi screen and pooled RNAi screen/selection based on different screening strategies. To date, several genome-wide RNAi screens have been successfully performed to identify host factors essential for influenza virus replication. However, the host factors identified by different research groups are not always consistent. Taking influenza virus screens as an example, we found that a number of screening parameters may directly or indirectly influence the primary hits identified by the screens. This review highlights the differences among the published genome-wide screening approaches and offers recommendations for performing a good pooled shRNA screen/selection.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Turkey 1 2%
Austria 1 2%
United Kingdom 1 2%
Canada 1 2%
Denmark 1 2%
Unknown 55 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 28%
Researcher 12 20%
Student > Bachelor 6 10%
Student > Postgraduate 5 8%
Student > Master 5 8%
Other 8 13%
Unknown 7 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 35%
Biochemistry, Genetics and Molecular Biology 17 28%
Medicine and Dentistry 5 8%
Immunology and Microbiology 4 7%
Engineering 2 3%
Other 3 5%
Unknown 8 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2015.
All research outputs
#17,285,668
of 25,373,627 outputs
Outputs from Journal of Clinical Bioinformatics
#33
of 61 outputs
Outputs of similar age
#164,491
of 271,801 outputs
Outputs of similar age from Journal of Clinical Bioinformatics
#4
of 4 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 61 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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