Title |
Hydrocortisone enhances the barrier properties of HBMEC/ciβ, a brain microvascular endothelial cell line, through mesenchymal-to-endothelial transition-like effects
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Published in |
Fluids and Barriers of the CNS, March 2015
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DOI | 10.1186/s12987-015-0003-0 |
Pubmed ID | |
Authors |
Tomomi Furihata, Shinya Kawamatsu, Ryo Ito, Kosuke Saito, Shota Suzuki, Satoshi Kishida, Yoshiro Saito, Atsuko Kamiichi, Kan Chiba |
Abstract |
Because in vitro blood-brain barrier (BBB) models are important tools for studying brain diseases and drug development, we recently established a new line of conditionally immortalized human brain microvascular endothelial cells (HBMEC/ciβ) for use in such models. Since one of the most important functional features of the BBB is its strong intercellular adhesion, in this study, we aimed at improving HBMEC/ciβ barrier properties by means of culture media modifications, thus enhancing their use for future BBB studies. In addition, we simultaneously attempted to obtain insights on related mechanistic properties. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | 2% |
Unknown | 49 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 22% |
Student > Master | 7 | 14% |
Student > Doctoral Student | 6 | 12% |
Student > Bachelor | 6 | 12% |
Researcher | 5 | 10% |
Other | 6 | 12% |
Unknown | 9 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 11 | 22% |
Agricultural and Biological Sciences | 8 | 16% |
Engineering | 5 | 10% |
Medicine and Dentistry | 4 | 8% |
Immunology and Microbiology | 3 | 6% |
Other | 8 | 16% |
Unknown | 11 | 22% |