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Mendeley readers
| Title |
Validation of Four Reference Genes for Quantitative mRNA Expression Studies in a Rat Model of Inflammatory Injury
|
|---|---|
| Published in |
Molecular Pain, January 2014
|
| DOI | 10.1186/1744-8069-10-55 |
| Pubmed ID | |
| Authors |
Roxanne Y Walder, Anne-Sophie Wattiez, Stephanie R White, Blanca Marquez de Prado, Marta V Hamity, Donna L Hammond |
| Abstract |
Real-time quantitative PCR (qPCR) is a technique frequently used to measure changes in mRNA expression. To ensure validity of experimental findings, it is important to normalize the qPCR data to reference genes that are stable and unaffected by the experimental treatment to correct for variability among samples. Unlike in some models of neuropathic pain, reference genes for models of inflammatory injury have not been validated. This study examined four candidate reference genes in an effort to identify and validate optimal genes for normalization of transcriptional changes occurring in the dorsal horn of the spinal cord and the rostral ventromedial medulla (RVM) following intraplantar injection of complete Freund's adjuvant (CFA). |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 34 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 23% |
| Researcher | 6 | 17% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Doctoral Student | 2 | 6% |
| Other | 1 | 3% |
| Other | 4 | 11% |
| Unknown | 10 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 14% |
| Neuroscience | 5 | 14% |
| Agricultural and Biological Sciences | 5 | 14% |
| Medicine and Dentistry | 3 | 9% |
| Business, Management and Accounting | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 13 | 37% |