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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Quantification of damage in DNA recovered from highly degraded samples – a case study on DNA in faeces
|
|---|---|
| Published in |
Frontiers in Zoology, August 2006
|
| DOI | 10.1186/1742-9994-3-11 |
| Pubmed ID | |
| Authors |
Bruce E Deagle, J Paige Eveson, Simon N Jarman |
| Abstract |
Poorly preserved biological tissues have become an important source of DNA for a wide range of zoological studies. Measuring the quality of DNA obtained from these samples is often desired; however, there are no widely used techniques available for quantifying damage in highly degraded DNA samples. We present a general method that can be used to determine the frequency of polymerase blocking DNA damage in specific gene-regions in such samples. The approach uses quantitative PCR to measure the amount of DNA present at several fragment sizes within a sample. According to a model of random degradation the amount of available template will decline exponentially with increasing fragment size in damaged samples, and the frequency of DNA damage (lambda) can be estimated by determining the rate of decline. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Saint Barthélemy | 1 | 17% |
| United Kingdom | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 5 | 83% |
| Members of the public | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 485 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 1% |
| United Kingdom | 5 | 1% |
| Netherlands | 2 | <1% |
| Germany | 2 | <1% |
| Canada | 2 | <1% |
| Italy | 1 | <1% |
| Australia | 1 | <1% |
| Malaysia | 1 | <1% |
| Chile | 1 | <1% |
| Other | 6 | 1% |
| Unknown | 457 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 112 | 23% |
| Researcher | 77 | 16% |
| Student > Master | 74 | 15% |
| Student > Bachelor | 53 | 11% |
| Student > Postgraduate | 22 | 5% |
| Other | 72 | 15% |
| Unknown | 75 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 249 | 51% |
| Biochemistry, Genetics and Molecular Biology | 62 | 13% |
| Environmental Science | 49 | 10% |
| Veterinary Science and Veterinary Medicine | 7 | 1% |
| Medicine and Dentistry | 6 | 1% |
| Other | 27 | 6% |
| Unknown | 85 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2023.
All research outputs
#4,867,428
of 23,914,787 outputs
Outputs from Frontiers in Zoology
#259
of 667 outputs
Outputs of similar age
#10,088
of 55,637 outputs
Outputs of similar age from Frontiers in Zoology
#1
of 2 outputs
Altmetric has tracked 23,914,787 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 667 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 21.2. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 55,637 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them