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Fluxome study of Pseudomonas fluorescens reveals major reorganisation of carbon flux through central metabolic pathways in response to inactivation of the anti-sigma factor MucA

Overview of attention for article published in BMC Systems Biology, February 2015
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Title
Fluxome study of Pseudomonas fluorescens reveals major reorganisation of carbon flux through central metabolic pathways in response to inactivation of the anti-sigma factor MucA
Published in
BMC Systems Biology, February 2015
DOI 10.1186/s12918-015-0148-0
Pubmed ID
Authors

Stina K Lien, Sebastian Niedenführ, Håvard Sletta, Katharina Nöh, Per Bruheim

Abstract

The bacterium Pseudomonas fluorescens switches to an alginate-producing phenotype when the pleiotropic anti-sigma factor MucA is inactivated. The inactivation is accompanied by an increased biomass yield on carbon sources when grown under nitrogen-limited chemostat conditions. A previous metabolome study showed significant changes in the intracellular metabolite concentrations, especially of the nucleotides, in mucA deletion mutants compared to the wild-type. In this study, the P. fluorescens SBW25 wild-type and an alginate non-producing mucA- ΔalgC double-knockout mutant are investigated through model-based (13)C-metabolic flux analysis ((13)C-MFA) to explore the physiological consequences of MucA inactivation at the metabolic flux level. Intracellular metabolite extracts from three carbon labelling experiments using fructose as the sole carbon source are analysed for (13)C-label incorporation in primary metabolites by gas and liquid chromatography tandem mass spectrometry.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Belgium 1 2%
Brazil 1 2%
Unknown 51 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 24%
Researcher 8 15%
Student > Bachelor 6 11%
Professor > Associate Professor 6 11%
Student > Master 5 9%
Other 6 11%
Unknown 10 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 30%
Biochemistry, Genetics and Molecular Biology 13 24%
Engineering 7 13%
Computer Science 3 6%
Immunology and Microbiology 2 4%
Other 1 2%
Unknown 12 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 April 2015.
All research outputs
#17,751,741
of 22,796,179 outputs
Outputs from BMC Systems Biology
#770
of 1,142 outputs
Outputs of similar age
#173,357
of 255,035 outputs
Outputs of similar age from BMC Systems Biology
#14
of 21 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,142 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 255,035 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.