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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Large-scale expansion of Wharton’s jelly-derived mesenchymal stem cells on gelatin microbeads, with retention of self-renewal and multipotency characteristics and the capacity for enhancing skin wound healing
|
|---|---|
| Published in |
Stem Cell Research & Therapy, March 2015
|
| DOI | 10.1186/s13287-015-0031-3 |
| Pubmed ID | |
| Authors |
Guifang Zhao, Feilin Liu, Shaowei Lan, Pengdong Li, Li Wang, Junna Kou, Xiaojuan Qi, Ruirui Fan, Deshun Hao, Chunling Wu, Tingting Bai, Yulin Li, Jin Yu Liu |
| Abstract |
Successful stem cell therapy relies on large-scale generation of stem cells and their maintenance in a proliferative multipotent state. This study aimed to establish a 3D culture system for large-scale generation of hWJ-MSCs and investigated the self-renewal activity, genomic stability and multi-lineage differentiation potential of such hWJ-MSCs in enhancing skin wound healing. hWJ-MSCs were seeded on gelatin microbeads and cultured in spinning bottles (3D cultures). Cell proliferation, karyotype analysis, surface marker expression, multipotent differentiation (adipogenic, chondrogenic, and osteogenic potentials), and expression of core transcription factors (OCT4, SOX2, NANOG, and C-MYC), as well as their efficacy in accelerating skin wound healing, were investigated and compared with those of hWJ-MSCs derived from 2D cultures, using in vivo and in vitro experiments. hWJ-MSCs attached to and proliferated on gelatin microbeads in 3D cultures reaching a maximum of 1.1-1.30 × 10(7)cells on 0.5 g of microbeads by days 8-14; in contrast, hWJ-MSCs derived from 2D cultures reached a maximum of 6.5 -11.5 × 10(5) cells per well in a 24-well plate by days 6-10. hWJ-MSCs derived by 3D culture incorporated significantly more EdU (P < 0.05) and had a significantly higher proliferation index (P < 0.05) than those derived from 2D culture. Immunofluorescence staining, real-time PCR, flow cytometry analysis, and multipotency assays showed that hWJ-MSCs derived from 3D culture retained MSC surface markers and multipotency potential similar to 2D culture-derived cells. 3D culture-derived hWJ-MSCs also retained the expression of core transcription factors at levels comparable to their 2D culture counterparts. Direct injection of hWJ-MSCs derived from 3D or 2D cultures into animals exhibited similar efficacy in enhancing skin wound healing. Thus, hWJ-MSCs can be expanded markedly in gelatin microbeads, while retaining MSC surface marker expression, multipotent differential potential, and expression of core transcription factors. These cells also efficiently enhanced skin wound healing in vivo, in a manner comparable to that of hWJ-MSCs obtained from 2D culture. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 72 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 18% |
| Researcher | 9 | 12% |
| Student > Bachelor | 8 | 11% |
| Student > Master | 7 | 10% |
| Student > Doctoral Student | 6 | 8% |
| Other | 9 | 12% |
| Unknown | 21 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 15% |
| Agricultural and Biological Sciences | 9 | 12% |
| Medicine and Dentistry | 8 | 11% |
| Engineering | 7 | 10% |
| Materials Science | 3 | 4% |
| Other | 10 | 14% |
| Unknown | 25 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2019.
All research outputs
#2,585,555
of 22,796,179 outputs
Outputs from Stem Cell Research & Therapy
#193
of 2,418 outputs
Outputs of similar age
#34,928
of 263,733 outputs
Outputs of similar age from Stem Cell Research & Therapy
#9
of 64 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,418 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,733 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.