Title |
Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome
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Published in |
Plant Methods, March 2015
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DOI | 10.1186/s13007-015-0062-x |
Pubmed ID | |
Authors |
Leonardo Galindo-González, David Pinzón-Latorre, Erik A Bergen, Dustin C Jensen, Michael K Deyholos |
Abstract |
Detection of induced mutations is valuable for inferring gene function and for developing novel germplasm for crop improvement. Many reverse genetics approaches have been developed to identify mutations in genes of interest within a mutagenized population, including some approaches that rely on next-generation sequencing (e.g. exome capture, whole genome resequencing). As an alternative to these genome or exome-scale methods, we sought to develop a scalable and efficient method for detection of induced mutations that could be applied to a small number of target genes, using Ion Torrent technology. We developed this method in flax (Linum usitatissimum), to demonstrate its utility in a crop species. |
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Unknown | 3 | 100% |
Demographic breakdown
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Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
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Japan | 1 | 2% |
Spain | 1 | 2% |
Norway | 1 | 2% |
Unknown | 55 | 95% |
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Researcher | 11 | 19% |
Student > Ph. D. Student | 11 | 19% |
Student > Master | 9 | 16% |
Student > Bachelor | 5 | 9% |
Student > Doctoral Student | 3 | 5% |
Other | 10 | 17% |
Unknown | 9 | 16% |
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Unspecified | 2 | 3% |
Other | 3 | 5% |
Unknown | 10 | 17% |