Title |
Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel
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Published in |
BMC Veterinary Research, January 2015
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DOI | 10.1186/s12917-015-0370-4 |
Pubmed ID | |
Authors |
Carlos E Giraldo, María E Álvarez, Jorge U Carmona |
Abstract |
There is a lack information on the effects of the most commonly used anticoagulants for equine platelet rich plasmas (PRPs) elaboration on cell counts and growth factor release from platelet rich gels (PRGs). The aims of this study were 1) to compare the effects of the anticoagulants sodium citrate (SC), acid citrate dextrose solution A (ACD-A) and ACD-B on platelet (PLT), leukocyte (WBC) and on some parameters associated to platelet activation including mean platelet volume (MPV) and platelet distribution width (PDW) between whole blood, pure PRP (P-PRP) and platelet-poor plasma (PPP); 2) to compare transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor isoform BB (PDGF-BB) concentrations in supernatants from pure PRG (P-PRG), platelet-poor gel (PPG), P-PRP lysate (positive control) and plasma (negative control); 3) to establish the possible correlations between all the studied cellular and molecular parameters. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 72 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 18% |
Student > Master | 12 | 17% |
Student > Bachelor | 10 | 14% |
Other | 6 | 8% |
Student > Postgraduate | 5 | 7% |
Other | 13 | 18% |
Unknown | 13 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 16 | 22% |
Veterinary Science and Veterinary Medicine | 12 | 17% |
Agricultural and Biological Sciences | 12 | 17% |
Biochemistry, Genetics and Molecular Biology | 7 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
Other | 6 | 8% |
Unknown | 17 | 24% |