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Mendeley readers
Attention Score in Context
| Title |
Acyclic retinoid induces differentiation and apoptosis of murine hepatic stem cells
|
|---|---|
| Published in |
Stem Cell Research & Therapy, March 2015
|
| DOI | 10.1186/s13287-015-0046-9 |
| Pubmed ID | |
| Authors |
Hong-Bin Guan, Yun-Zhong Nie, Yun-Wen Zheng, Kazuya Takiguchi, Hong-Wei Yu, Ran-Ran Zhang, Bin Li, Tomonori Tsuchida, Hideki Taniguchi |
| Abstract |
Therapeutic potential of acyclic retinoid (ACR), a synthetic retinoid, has been confirmed in experimental and clinical studies. Therapeutic targets include precancerous and cancer stem cells. As ACR is also involved in developmental processes, its effect on normal hepatic stem cells (HpSCs) should be investigated for understanding the underlying mechanisms. Here, we examined effects of the acyclic retinoid peretinoin on fresh isolated murine HpSCs. We isolated c-kit(-)CD29(+)CD49f(+/low)CD45(-)Ter119(-) cells from murine fetal livers using flow cytometry. To evaluate the effect of ACR, we traced clonal expansion and analyzed cell differentiation as well as apoptosis during the induction process by immunofluorescent staining and marker gene expression. ACR dose-dependently inhibited HpSCs expansion. Stem cell clonal expansion was markedly inhibited during the culture period. Moreover, ACR showed a significant promotion of HpSC differentiation and induction of cellular apoptosis. The expression of stem cell marker genes, Afp, Cd44, and Dlk, was downregulated, while that of mature hepatocyte genes, Alb and Tat, and apoptosis-related genes, Annexin V and Caspase-3, were upregulated. Flow cytometry showed that the proportion of Annexin V-positive cells increased after ACR incubation compared with the control. Data obtained by immunofluorescent staining for albumin and Caspase-3 corroborated the data on gene expression. Finally, we found that ACR directly regulates the expression of retinoic acid receptors and retinoid X receptors. These findings indicate that ACR inhibits the clonal expansion of normal HpSCs in vitro and promotes the differentiation of immature cells by regulating receptors of retinoic acid. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 38% |
| Student > Master | 2 | 25% |
| Researcher | 1 | 13% |
| Professor > Associate Professor | 1 | 13% |
| Unknown | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 25% |
| Agricultural and Biological Sciences | 2 | 25% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
| Chemical Engineering | 1 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 1 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2015.
All research outputs
#4,172,589
of 22,796,179 outputs
Outputs from Stem Cell Research & Therapy
#412
of 2,418 outputs
Outputs of similar age
#53,008
of 263,459 outputs
Outputs of similar age from Stem Cell Research & Therapy
#15
of 66 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,418 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,459 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 66 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.