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Biphasic monopolar electrical stimulation induces rapid and directed galvanotaxis in adult subependymal neural precursors

Overview of attention for article published in Stem Cell Research & Therapy, April 2015
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  • Good Attention Score compared to outputs of the same age (74th percentile)

Mentioned by

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6 tweeters
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1 peer review site

Citations

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25 Dimensions

Readers on

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85 Mendeley
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Title
Biphasic monopolar electrical stimulation induces rapid and directed galvanotaxis in adult subependymal neural precursors
Published in
Stem Cell Research & Therapy, April 2015
DOI 10.1186/s13287-015-0049-6
Pubmed ID
Authors

Robart Babona-Pilipos, Alex Pritchard-Oh, Milos R Popovic, Cindi M Morshead

Abstract

Following injury such as stroke, adult mammalian subependymal neural precursor cells (NPCs) are induced to proliferate and migrate toward the lesion site where they differentiate into neural cells, albeit with limited efficacy. We are interested in enhancing this migratory ability of NPCs with the long-term goal of promoting neural repair. Herein we build on our previous studies demonstrating that direct current electric fields (DCEFs) promote rapid and cathode-directed migration of undifferentiated adult NPCs (but not differentiated phenotypes) - a phenomenon known as galvanotaxis. While galvanotaxis represents a promising strategy to promote NPC recruitment to lesion sites, stimulation of neural tissue with DCEFs is not a clinically-viable strategy due to the associated accumulation of charge and toxic byproducts. Balanced biphasic waveforms prevent the accumulation of charge and thus are outside of the limitations of DCEFs. In this study, we investigated the effects of balanced biphasic electrical stimulation on the migratory behaviour of undifferentiated subependymal NPCs and their differentiated progeny. NPCs were isolated from the subependymal zone of adult mouse brains and cultured in a NPC colony-forming assay to form neurospheres. Neurospheres were plated onto galvanotaxis chambers in conditions that either promoted maintenance in an undifferentiated state or promoted differentiation into mature phenotypes. Chambers containing cells were then time-lapse imaged in the presence of either biphasic monopolar, or biphasic bipolar electrical stimulation, or in the complete absence of electrical stimulation. Single cell migration was subsequently tracked and the cells' magnitude of velocity, directedness and tortuosity were quantified. We demonstrate, for the first time, the use of balanced biphasic electric fields to induce galvanotaxis of NPCs. Undifferentiated adult mouse subependymal NPCs exposed to biphasic monopolar stimulation undergo rapid and directed migration toward the cathode. In contrast, both biphasic bipolar stimulation and the lack of electrical stimulation produced non-directed migration of NPCs. Notably, NPCs induced to differentiate into mature phenotypes prior to exposure to electrical stimulation do not migrate in the presence or absence of biphasic stimulation. We purport that balanced biphasic stimulation represents a clinically-viable technique for mobilizing NPCs that may be integrated into strategies for promoting endogenous neurorepair.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Switzerland 1 1%
Unknown 84 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 19 22%
Researcher 13 15%
Student > Ph. D. Student 10 12%
Student > Master 8 9%
Other 5 6%
Other 15 18%
Unknown 15 18%
Readers by discipline Count As %
Engineering 17 20%
Neuroscience 13 15%
Medicine and Dentistry 13 15%
Agricultural and Biological Sciences 9 11%
Biochemistry, Genetics and Molecular Biology 5 6%
Other 7 8%
Unknown 21 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 October 2020.
All research outputs
#4,910,887
of 19,040,944 outputs
Outputs from Stem Cell Research & Therapy
#435
of 1,961 outputs
Outputs of similar age
#60,404
of 237,575 outputs
Outputs of similar age from Stem Cell Research & Therapy
#2
of 3 outputs
Altmetric has tracked 19,040,944 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 1,961 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 237,575 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.