Title |
Improved genome annotation through untargeted detection of pathway-specific metabolites
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Published in |
BMC Genomics, June 2011
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DOI | 10.1186/1471-2164-12-s1-s6 |
Pubmed ID | |
Authors |
Benjamin P Bowen, Curt R Fischer, Richard Baran, Jillian F Banfield, Trent Northen |
Abstract |
Mass spectrometry-based metabolomics analyses have the potential to complement sequence-based methods of genome annotation, but only if raw mass spectral data can be linked to specific metabolic pathways. In untargeted metabolomics, the measured mass of a detected compound is used to define the location of the compound in chemical space, but uncertainties in mass measurements lead to "degeneracies" in chemical space since multiple chemical formulae correspond to the same measured mass. We compare two methods to eliminate these degeneracies. One method relies on natural isotopic abundances, and the other relies on the use of stable-isotope labeling (SIL) to directly determine C and N atom counts. Both depend on combinatorial explorations of the "chemical space" comprised of all possible chemical formulae comprised of biologically relevant chemical elements. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 4 | 7% |
Brazil | 1 | 2% |
Switzerland | 1 | 2% |
Denmark | 1 | 2% |
United Kingdom | 1 | 2% |
Unknown | 49 | 86% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 22 | 39% |
Student > Ph. D. Student | 15 | 26% |
Other | 2 | 4% |
Professor > Associate Professor | 2 | 4% |
Student > Master | 2 | 4% |
Other | 4 | 7% |
Unknown | 10 | 18% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 26 | 46% |
Chemistry | 6 | 11% |
Biochemistry, Genetics and Molecular Biology | 6 | 11% |
Engineering | 2 | 4% |
Nursing and Health Professions | 1 | 2% |
Other | 5 | 9% |
Unknown | 11 | 19% |