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Mendeley readers
Attention Score in Context
| Title |
High yield purification of Helicobacter pylori neutrophil-activating protein overexpressed in Escherichia coli
|
|---|---|
| Published in |
BMC Biotechnology, April 2015
|
| DOI | 10.1186/s12896-015-0136-x |
| Pubmed ID | |
| Authors |
Yu-Chi Yang, Ting-Yu Kuo, Zhi-Wei Hong, Han-Wen Chang, Chung-Chu Chen, Te-Lung Tsai, Hua-Wen Fu |
| Abstract |
Helicobacter pylori neutrophil-activating protein (HP-NAP) is involved in H. pylori-induced gastric inflammation. Due to its immunogenic and immunomodulatory properties, HP-NAP has been used for developing vaccines against H. pylori infection and new drugs for cancer therapy. Here, we provide a simple process for high-yield production of HP-NAP by applying one-step negative chromatography to purify recombinant HP-NAP expressed in Escherichia coli (E. coli). In our E. coli expression system, recombinant HP-NAP constitutes nearly 70% of the total protein. Overexpressed recombinant HP-NAP is almost completely soluble upon cell lysis at pH 9.5. Under the optimal condition at pH 8.0, recombinant HP-NAP with purity higher than 95% can be obtained from E. coli by collecting the unbound fraction using diethylaminoethyl (DEAE) Sephadex resin in batch mode. The overall yield of HP-NAP from a 50-ml E. coli culture is ~ 19 mg. The purified HP-NAP folds into a multimer with a secondary structure of α-helix and is able to trigger the production of reactive oxygen species by neutrophils. Purification of recombinant HP-NAP overexpressed in E. coli using DEAE Sephadex negative mode batch chromatography is an efficient method for high-yield production of highly pure HP-NAP in its native state. The purified HP-NAP is useful for various clinical applications including vaccine development, diagnosis, and new drug development. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 24% |
| Researcher | 2 | 12% |
| Student > Ph. D. Student | 2 | 12% |
| Other | 1 | 6% |
| Student > Doctoral Student | 1 | 6% |
| Other | 3 | 18% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Medicine and Dentistry | 3 | 18% |
| Agricultural and Biological Sciences | 2 | 12% |
| Immunology and Microbiology | 2 | 12% |
| Veterinary Science and Veterinary Medicine | 1 | 6% |
| Other | 2 | 12% |
| Unknown | 4 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 April 2015.
All research outputs
#4,172,977
of 22,799,071 outputs
Outputs from BMC Biotechnology
#213
of 935 outputs
Outputs of similar age
#53,379
of 264,940 outputs
Outputs of similar age from BMC Biotechnology
#20
of 22 outputs
Altmetric has tracked 22,799,071 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 935 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,940 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.